周围神经损伤对骨折愈合过程中CGRP表达的实验研究

目的 通过建立选择性切断大鼠感觉/运动神经联合胫骨骨折的动物模型,检测周围神经损伤对骨折愈合过程中CGRP表达变化的影响,为治疗骨不愈合及延迟愈合提供理论依据及实验基础.方法 Wistar大鼠90只分为3组,每组30只,前根运动神经切断+胫骨骨折组(ART组)、后根感觉神经切断+胫骨骨折组(PRT组)、单纯胫骨骨折组(...

Experimental Study of CGRP Changes in Peripheral Nerve Injury during Fracture Healing Process

Objective To study the calcitonin gene-related peptide（CGRP）expression during fracture healing in relation with peripheral nerve injury by way of the selective cutting of rats＇ sensory/motor nerve combined with tibial fracture made as animal models,providing theoretical basis about non-union and delayed union of fracture healing.Methods 60 Wistar rats divided into 3 different groups：ventral motor nerve root＋tibial fracture group（anterior rhizotomy group,ART group）;dorsal sensory nerve root＋tibial fracture group（posterior rhizotomy group,PRT group）;simple tibial fracture group（Sham operated group,SO group）,which were killed 7 days,14 days,21 days,28 days postoperatively,and later the callus specimens from 5 mm above and below the fracture site were obtained,the CGRP expression during fracture healing with peripheral nerve injury were observed.Results In SO group,7 days after fractures,CGRP positive nerve fibers can be seen within the callus,distributed along the blood vessels,14-21 days a large number of CGRP-positive nerve fibers seen along the woven bone edge,28 days still got more.In ART group,7 days after fractures,CGRP decreased and showed positive expression,its expression achieved to the lowest 14 days,21 days later.In PRT group,besides the 7 days after fractures that showed positive expression,the other observed durations only showed low levels of CGRP.The differences between SO group,ART group and PRT group were significant 14 days and 21 days postoperatively（P0.05）.Conclusion In fractures healing process,the effect of sensory nerve fibers to the healing process is more significant than the motor nerve fibers.Denervation of the nerves（especially the sensory nerves）is not conducive to fracture healing,complete innervation is necessary for normal fracture healing.