小针高频电凝配合注射肉毒杆菌毒素A诱导家兔咬肌萎缩的实验研究

目的 探讨小针高频电凝配合肉毒毒素A治疗咬肌肥大的可行性.方法 健康家兔36只,随机分为4组,每组9只;A组在咬肌注射肉毒毒素A 2 U/kg;B组于咬肌插入数枚电凝针,20 W电凝3 s;C组为A组注射剂量为1 U/kg,联合高频电凝20 W,3 s;D组为空白对照组.A组、B组、C组治疗对侧注射等量生理盐水做自身对...

Experiment of Rabbit Masseter Muscle Atrophy Induced by High-frequency Electric Coagulation of Small Needle with Injection of Botulinum Toxin A

Objective To investigate a new method of minimally invasive treatment of benign masseteric hypertrophy. Methods Thirty six rabbits were randomly divided into 4 groups,Group A（n =9）was injected 2 U/kg botulinum toxin on rabbit masseter muscle ;group B （n = 9）was injected several pieces of electro coagulation needles on the masseter muscle; group C（n =9）was treated together with 1 U/kg botulinum toxin and high-frequency electro coagulation 20 W,3 seconds; group D（n =9）was the control group. All the rabbits in group A,group B and group C were injected with nomal saline on the opposite side After 4 weeks,8 weeks and 12 weeks,the masseter muscle was evaluated by naked eye observation,the wet weigh was measured, the thickness by B Type Uhrasonography and their changes in its structure were observed under staining microscope,and the effect on the masseter muscle atrophy was analyzed. Results The thickness of masseter muscle in group A,B,C reduced and the wet weight decreased,the muscle atrophy in group C reduced most obviously（P 〈0.05）. The Muscle fiber became thin and turned to be tighter arrangement,while the muscle in coagulation area there were necrotic tissue. Conclusion The muscle fiber could become much thinner and tight arrangement, and even induce necrosis under high-frequency electro coagulation with botulinum toxin A injection. Masseter muscle becomes thin,wet weight decreases,and atrophy of muscle tissue could provide a new experimental study for the clinical treatment of benign masseteric hypertrophy.