Comparison of gene expression between metastatic derivatives and their poorly metastatic parental cells implicates crucial tumor-environment interaction in metastasis of head and neck squamous cell carcinoma |
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Authors: | Chen Zhuo Zhang Kun Zhang Xin Yuan Xiao H Yuan Zhenyu Jin Li Xiong Momiao |
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Institution: | (1) Department of Head and Neck Surgery, The University of Texas M.D. Anderson Cancer Center, The University of Texas at Houston School of Public Health, Houston, Texas, USA;(2) Human Genetics Center, The University of Texas at Houston School of Public Health, Houston, Texas, USA |
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Abstract: | Metastasis of human head and neck cancer is a multistep and highly heterogeneous process requiring activation and deactivation
of multiple and specific genes. To identify these genes, we established highly metastatic head and neck squamous cell carcinoma
(HNSCC) cell lines from poorly metastatic HNSCC cells through in vivo selection using a lymph node metastatic mouse model. The very close genetic relationship between these highly metastatic
cell lines and the parental cell line provided an excellent model for differential gene expression analysis using cDNA microarrays.
Comparison of 6 cell lines established individually from the lymph node metastases with their poorly metastatic parental cell
line revealed 33 differentially expressed genes. Some of these genes are involved in cellular signal transduction and matrix
modeling. Differences in expression of members of the tumor necrosis factor, interleukin, caspase, and matrix metalloproteinase
families were also examined. We found that two upregulated genes participated in the NF-κB regulatory pathway. Furthermore,
differences in gene expression between six cell lines derived from primary tumors and six cell lines derived from lymph node
metastases in the mouse model were analyzed statistically. Tissue growth factor-β and tumor necrosis factor-related genes
showed significantly altered expression in cells derived from lymph node metastases as compared with cells derived from primary
tumors, suggesting that the differential growth advantage of metastatic cells requires more aggressive responses to their
environment, such as a lymph node tissue.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | apoptosis cDNA microarray inflammation metastasis and squamous cell carcinoma |
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