VEGF-C在乳腺癌组织中的表达及临床意义

目的检测血管内皮生长因子C(VEGF-C)在乳腺癌、乳腺良性肿瘤及正常乳腺组织中的表达,探讨VEGF-C与乳腺癌淋巴结转移及预后的关系。方法选用10例正常乳腺组织、30例乳腺良性肿瘤及60例乳腺恶性肿瘤标本,以免疫组织化学及半定量反转录PCR(RT-PCR)检测VEGF-C的表达。结果在乳腺恶性肿瘤组织中,VEGF-C阳性表达率为46.7%,明显高于正常乳腺(10.0%)和乳腺良性肿瘤组织(13.3%)(P<0.01);乳腺癌中VEGF-C的阳性表达率为48.2%,明显高于乳腺叶状囊肉瘤(25.0%)(P<0.01);在伴有淋巴结转移的乳腺癌组织中,VEGF-C的阳性表达率(55.0%)明显高于没有淋巴结转移的乳腺癌组织(30.0%)(P<0.01)。结论VEGF-C在乳腺癌组织中表达明显增高,并与乳腺癌的淋巴结转移显著相关,可作为乳腺癌转移、复发的预测指标之一。

Expression and significance of VEGF - C in malignant mammary neoplasms

Objective We examined the expression of VEGF-C in mammary neoplasm and normal breast tissue to determine its role in the progression of breast cancer. Methods Protein and mRNA of VEGF-C in 10 cases of normal breast tissue, 30 cases of benign breast tumor and 40 cases of malignant breast tumor were detected by immunohistochemistry staining or half-quantity RT-PCR. Results Positive rate of VEGF-C in malignant breast tumor was 46.7%,much higher than that in normal breast tissue and benign breast tumor, which were 10.0% and13.3%, respectively. VEGF-C positive rate in breast cancer and cystosarcoma were 48.2%and 25.0%, there was significant difference between the two groups(P<0.01);Similarly, the positive expression of VEGF-C in breast cancer with lymph node metastasis was much higher than that in breast cancer without lymph node metastasis. Conclusion The expression of VEGF-C in breast cancer is significantly elevated and is related with lymph node metastasis. It may be a maker to predict the metastasis and recurrence of breast cancer.