| HPLC法测定毛冬青颗粒中腺苷的含量 |
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| 引用本文： | 陈怡禄,卓仪,邓六勤,周启仲,郭穗霞,欧阳珊. HPLC法测定毛冬青颗粒中腺苷的含量[J]. 中国中医药现代远程教育, 2009, 7(3): 110-111. DOI: 10.3969/j.issn.1672-2779.2009.03.027 |
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| 作者姓名： | 陈怡禄 卓仪 邓六勤 周启仲 郭穗霞 欧阳珊 |
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| 作者单位： | 广州市儿童医院,510120 |
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| 基金项目： | 广东省科技厅资助项目 |
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| 摘 要： | 目的建立毛冬青颗粒中腺苷的高效液相测定方法。方法采用HPLC法测定腺苷的含量。KromasilC-18柱（4．6mm×250mm，5μm），以磷酸盐缓冲液（pH6．5）．甲醇（17：3）为流动相，柱温为室温，流速为1ml·min^-1，检测波长为260nm。结果腺苷在0．18μg～0．90μg范围内与其峰面积呈良好的线性关系（r=0．9997，n=5），平均回收率为98．5％（RSD=1．28％）。结论该测定方法精密度高，重现性好，可用于毛冬青颗粒的质量控制。
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| 关 键 词： | 毛冬青颗粒 腺苷 高效液相色谱 |
Content Determination of Ribosidoadenine in Maodongqing Keli by HPLC |
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| Chen Yilu,Zhuo Yi,Deng Liuqin,Zhou Qizhong,Guo Suixia,Ouyang Shan. Content Determination of Ribosidoadenine in Maodongqing Keli by HPLC[J]. Chinese Medicine Modern Distance Education of China, 2009, 7(3): 110-111. DOI: 10.3969/j.issn.1672-2779.2009.03.027 |
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| Authors: | Chen Yilu Zhuo Yi Deng Liuqin Zhou Qizhong Guo Suixia Ouyang Shan |
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| Affiliation: | Chen Yilu, Zhuo Yi ,Deng Liuqin, Zhou Qizhong, Guo Suixia, Ouyang San. (Guangzhou Children＇s Hospital, Guangzhou 510120) |
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| Abstract: | Objective To establish the determination of Ribosidoadenine in Maodongqing Keli. Methods The determination was carded out by HPLC with a KromasilC-18 （ 150mm × 4.6mm, 5μm） column, Phosphate buffered solution： Methanol （17：3） as a mobile phase, detection wavelength was at 260nm. Results Ribosidoadenine showed a good linear relationship at the range of 0.18μg-0.90μg（r=0.9997, n=5）, The average recovery of Ribosidoadenine was 98.5%（RSD= 1.28%）. Conclusion The method is insensitive, accurate, reproducible, specific and can be used for quality control of Maodongqing Keli. |
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| Keywords: | Maodongqing Keli Ribosidoadenine HPLC |
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