人微小病毒B19致胎肝造血细胞凋亡及其基因表达的意义

目的：研究人微小病毒Ｂ１９（ＨＰＶＢ１９）引致胎肝造血细胞凋亡及凋亡相关基因表达的变化，初步探讨ＨＰＶＢ１９引起死胎的机理．方法：采用原位末端标记法对２５例石蜡包埋死胎肝脏组织（其中ＨＰＶＢ１９ ＤＮＡ阳性者１０例，ＨＰＶＢ１９ ＤＮＡ阴性者１５例）进行细胞凋亡检测，并用免疫组化方法检测ｂｃ１－２，ｂａｘ表达情况．结果：１０例ＨＰＶＢ１９ ＤＮＡ阳性死胎肝脏组织中８例明显可见造血细胞的凋亡，６例b

Significance of apoptosis and its gene expression in hematopoietic cells of fetal liver induced by human parvovirus B19

AIM:To investigate the apoptosis and its gene expression in hematopoietic cells of fetal liver. METHODS: In situ end labeling (ISEL) was used to detect the apoptosis in 25 paraffin embedded fetal liver tissue samples and immunohistochemical method was used to investigate the expression of the bcl 2 and bax gene. RESULTS: Apoptosis was observed in 8 HPVB19 DNA positive cases (8/10), and the expression of bax was present in 6 cases(6/10). Apoptosis was only found in 2 HPVB19 DNA negative cases and the expression of bax was also present in 2 cases. But the expression of bcl 2 was found in none of the 25 cases. CONCLUSION: HPVB19 may induce apoptosis of hematopoietic cells in fetal liver and the virus may cause fetal death by this way.