细胞因子Ⅱ在遗传性视网膜色素变性的转基因小鼠视网膜的表达研究

目的 探讨细胞因子Ⅱ(ealpain Ⅱ)在视网膜色素变性过程中表达的变化。以此窥视遗传性视网膜色素变性的转基因小鼠(rds小鼠)发病的某些可能因素。方法 以C3B小鼠为对照，选择不同生长时期的rds小鼠的视网膜组织，用免疫组化方法检测CalpainlI在不同生长阶段的小鼠模型视网膜中的是否表达及表达情况。结果 1周即可见CalpainlI在rds小鼠视网膜神经节细胞层的表达，2周左右内核层也见表达，4周后外核层也开始出现由强至弱的ealpainⅡ强阳性表达，最终至全部视网膜剩余组织中均可见ealpainⅡ的表达。结论 只有高钙才能激活ealpainⅡ的表达，研究中ealpainⅡ参与了rds小鼠视网膜细胞的变性死亡。因此，高钙是rds小鼠视网膜变性的一个可能因素。

The expression of calpain

ObjectiveTo study some possible apoptosis mechanisms of the process of retinal degeneration in rds mice.MethodsThe retina of rds and C3B mice were collected at different time periods to form the experimental and control groups respectively.By using immunohistochemical technique,the expressions of calpain in the retina of rds and C3B mice at different time periods were analyzed.ResultsThe expression of calpain could be seen in the ganglionic layer of rds mice in the early stage(1 week),on the inner nuclear at about 2 weeks, outer nuclear layer at 4～5 weeks.The intense and scope increase with the growth of retina.After 4 weeks,the expression of calpain Ⅱ showed all of the rest of retina.ConclusionHigh calcium concentration activated the expression of Calpain Ⅱ.Therefore,high calcium concentration may be a factor in increasing the apoptosis of the retinal cells in the rds mice.The apoptosis may start from the ganglion cell layer in the rds mice.