| Abstract: | The physicochemical properties of the liver lysosomes were investigated in rats after administration of a single dose of the lysosomotropic agent Triton WR 1339 to intact rats and to rats with acute toxic hapatitis. Administration of the detergent to intact animals was followed by a decrease in the buoyant density of the particles, solubilization of the lysosomal enzymes, and reduced resistance of the particles in a hypotonic medium. Signs of overloading of the vacuolar apparatus with the detergent also were found in the liver cell lysosomes of rats with toxic hepatitis. The most marked solubilization of  -galactosidase, acid RNase, and cathepsin D was observed in the case of combined administration of CCl4 followed by WR 1339 24, 48, and 72 h and 7 days later. The possible consequences of overloading of the vacuolar apparatus of the rat liver cells are discussed.Central Research Laboratory, Novosibirsk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 87, No. 3, pp. 234–237, March, 1979. |
