斑马鱼3D解剖互动系统的制作

目的制作斑马鱼三维(3D)解剖互动系统软件。方法采用光学显微镜和微计算机断层扫描技术(Micro-CT)研究斑马鱼的组织构造,并收集其形态结构图像。使用Photoshop对获得的连续切片(HE染色)的图片进行校正,使用Autodesk 3ds Max 2012 64-bit-English软件进行三维模型建立。结果获得了斑马鱼骨骼的3D成像和连续切片图像(雄鱼144张,雌鱼179张),实现了斑马鱼重要器官的3D重构,建立了斑马鱼3D解剖互动系统(V 1.0),获得了计算机软件著作权登记证书(证书号:软著登字第0989966号)。结论斑马鱼3D解剖互动系统软件制作的完成为研究斑马鱼立体形态学奠定了基础。

Establishment of the three-dimensional anatomic interaction system of zebrafish (Danio rerio)

Objective The aim of this paper was to make a software of three-dimension (3D) anatomical interaction system in zebrafish (Danio rerio). Methods The structure of zebrafish was observed by light microscopy and Micro-CT, and the images of its morphological structure were collected. The pictures of serial section (HE-stained) were corrected by Photoshop. The 3D model was established by the software of Autodesk 3ds Max. Results The results showed the zebrafish skeletal 3D imaging and serial section images (male: 144, female: 179). We realized the 3D reconstruction of the vital organs of zebrafish, and established the 3D anatomy interaction system zebrafish (V 1.0), which obtained the computer software copyright registration certificate (Certificate Number: soft word board the Di_0989966). Conclusion The zebrafish 3D anatomy interaction system software afforded the three-dimensional morphological basis for the study of zebrafish, and was helpful to histomorphology recognization for researchers.