黄芪、山茱萸对肾炎小鼠尿蛋白谱的影响

目的：研究黄芪、山茱萸对肾炎小鼠尿蛋白谱的影响,旨在探讨中药治疗肾炎的分子学机制。方法：给小鼠注射右旋糖苷,造成慢性肾炎模型。动物随机分为黄芪组(灌胃,20 g·kg^-1·d^-1)、山茱萸组(灌胃,10 g·kg^-1·d^-1)、模型对照组(灌胃饮用水),另设正常对照组。造模同时给药,每天1次,连续12周。末次给药后,收集各组动物24 h尿液,分别用非浓缩尿液和冷冻干燥尿液作为样本,采用微流控芯片分析技术,对各实验组小鼠的尿样蛋白质进行分离、分析,比较各组动物尿样蛋白谱的差异。结果：模型对照组动物尿样中的蛋白质种类明显多于正常对照组,相对分子质量大于43×10³的蛋白种类和相对含量均明显增加；给予黄芪、山茱萸治疗的动物尿蛋白种类明显减少,尤其是相对分子质量大于50×10³的蛋白质明显减少,尿蛋白谱有明显改变,趋近于正常对照组的图谱。同时发现非浓缩尿样蛋白质以相对分子质量29,32,43,52,68,76×10³为主,而浓缩尿样蛋白质主要为相对分子质量22,24,32,46×10³的蛋白质,后者相对分子质量大于50×103的蛋白质明显减少。结论：黄芪和山茱萸具有降低肾炎小鼠尿中蛋白含量和种类的作用,提示两药具有保护肾脏的作用。

Effects of Radix Astragali and Fructus Corni on urinary protein pattern in nephropathy mice by microfluidic chip

OBJECTIVE: To study the urinary protein patterns of nephropathy mice induced by dextran and the effects of aquesous extract of Fructus Corni (AEFC) and Radix Astragali (AERA). METHOD: Nephropathy model was established by administrated with dextran to mice. Some of the dextran treated mice were given AERA (20 g x kg(-1) x d(-1)) as AERA group, other mice were given AEFC (10 g x kg(-1) x d(-1)) as AEFC group. Some of the dextran treated mice were given water as model group, some normal mice as normal control group. After a 12 weeks' treatment, 24 hour urine of four groups was collected, respectively. Each urinary sample was divided into two parts, one was non-concentrated urine sample, another was used as concentrated urine sample. Two kinds of urinary sample of four groups were analyzed with microfluidic chips on Agilent 2100 Bioanalyzer instrument. RESULT: Each group's urinary protein patterns were obtained, more than 20 proteins were were detected. Compared with normal group, about five kinds of protein were found in urinary sample of model group, among which M > 43 x 10(3) proteins were increased. Compared with model group, significant treated-related protein's kind and quantitative changes in AERA treated group and AEFC group were found. Urinary protein kinds were reduced, especially certain the proteins (M > 50 x 10(3)) were significantly decreased approach to normal patterns. Non-concentrated urine samples' protein pattern mainly included were proteins (M=29, 32, 43, 52, 68, 76 x 10(3) and concentrated urine samples mainly included the proteins (M=22, 24, 32, 46 x 10(3)). CONCLUSION: AERA and AEFC could reduce the urinary protein and made protein pattern different, which showed that radix astragali and fructus corni could play an important role in protecting renal function of nephropathy mice and finding the target protein markers related to AERA and AEFC effects on nephropathy mice.