慢性HBV感染者肝组织中BMP-7的表达与炎症纤维化的关系

目的研究慢性乙型肝炎病毒（HBV）感染者肝组织中骨形态发生蛋白7（BMP-7）表达与肝纤维化和炎症关系，初步探讨BMP-7慢性肝炎肝纤维化发生过程中可能发挥的作用。方法对81例慢性HBV感染者肝组织进行常规组织学观察，按病理肝纤维化和炎症分级进行分组。其中炎症GO级8例，G1级14例，G2级19例，G3级22例，G4级18例；纤维化SO级8例，S1级16例，S2级21例，S3级24例，S4级12例。肝组织进行BMP-7免疫组织化学染色和进行定量图像分析，比较各组中BMP-7达阳性单位的差异。结果慢性HBV感染者肝组织中BMP-7表达在随着炎症和纤维化严重程度的增加而增多；肝脏重度炎症时，肝组织中BMP-7表达明显增加，与纤维化程度无关；同样肝脏重度纤维化时，肝组织中BMP-7表达明显增加，与炎症程度无关。结论BMP-7在慢性肝炎肝纤维化发生过程中可能发挥抗炎和抗纤维化作用。

The relationship of expression of BMP-7 in the liver and hepatic inflammation and fibrosis in patients with chronic HBV infection

Objective To evaluate the relationship of the expression of bone morphogenefic protein7 (BMP-7) in the liver and the liver fibrosis and inflammation in patients with chronic hepatitis B virus (HBV) infection,to explore the role of BMP-7 in the fibrogenesis of chronic hepatitis B.Methods 81 patients chronically infected with HBV were enrolled.Liver biopsy were performed in all these patients.The hematoxylin staining and reticular fiber staining were performed for the grading of inflammation and staging of fibrosis,respectively.The patients were divided into different groups by the inflammatory grade or fibrosis stage (8,14,19,22,18 patients with inflammatory grade of G0,G1,G2,G3,G4,respectively;8,16,21,24,12 patients with fibrosis stage of S0,S1,S2,S3,S4,respectively).Then the immunohistochemical staining by BMP-7 were performed.The expression of BMP-7 in the liver was evaluated by digital image quantitative analysis system.We compared the expression of BMP-7 in the liver with different inflammatory grade and fibrosis stage.Results The expression of BMP-7 in the liver tissue increased with the increase of hepatic inflammatory grade and fibrosis.When liver biopsy showed a severe intrahepatic inflammation,the expression of BMP-7 significantly increased,regardless the intrahepatic fibrosis stage.Similarly,when liver biopsy showed a severe intrahepatic fibrosis,the expression of BMP-7 also significantly increased,regardless the intrahepatic inflammatory grade.Conclusion BMP-7 may play an important role as anti-inflammatory and anti-fibrogenic effect in the fibrogenesis of chronic hepatitis B.