| 补骨脂素对MCF-7/ADR耐药细胞内ADR及Ca2+浓度的影响 |
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| 引用本文： | 蔡天革,蔡宇,余绍蕾,冯笑珍.补骨脂素对MCF-7/ADR耐药细胞内ADR及Ca2+浓度的影响[J].航天医学与医学工程,2007,20(5):313-316. |
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| 作者姓名： | 蔡天革 蔡宇 余绍蕾 冯笑珍 |
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| 作者单位： | 1. 辽宁大学生命科学学院,辽宁沈阳 110036,中国
2. School of Pharmaceutical Science, Ji'nan University, Guangzhou Guangdong 510632 |
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| 基金项目： | National Science Fund Committee
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Liaoning State of Education
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广东省自然科学基金
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Guangdong State of Chinese Medicine |
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| 摘 要： | 目的 研究补骨脂素对MCF-7/ADR耐药细胞逆转作用及对耐药细胞内Ca2 浓度影响,探讨其可能作用机制.方法 采用MTT法测定补骨脂素对细胞增殖抑制作用,高效液相色谱法检测细胞内ADR的浓度,激光共聚焦显微镜测定细胞内不同时段的Ca2 浓度.结果 补骨脂素在1～20 μmol/L浓度下能不同程度降低ADR对MCF-7/ADR细胞的IC50,并不同程度提高细胞内ADR浓度;1～20 μmol/L补骨脂素在不同作用时段(24、48、96 h)对MCF-7/ADR细胞内Ca2 浓度与作用时间呈负相关.结论 补骨脂素能逆转MCF-7/ADR细胞的MDR,其作用机制与影响耐药细胞内Ca2 浓度,故而增加细胞内ADR浓度有关.
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| 关 键 词： | 补骨脂素 多药耐药 逆转 MCF-7/ADR细胞 psoralen multidrug resistance reverse MCF-7/ADR cells 补骨脂素 耐药 胞内 浓度的影响 Multidrug Resistance Effects Concentration mechanism related increase reverse negative correlation accumulation value Results concentrations different duration confocal microscope HPLC |
| 文章编号： | 1002-0837（2007）05-0313-04 |
| 修稿时间： | 2007-07-09 |
Effects of Psoralen on ADR Multidrug Resistance and Ca2+ Concentration in MCF-7/ADR Cells |
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| CAI Tian-ge,CAI Yu,YU Shao-lei,FENG Xiao-zhen.Effects of Psoralen on ADR Multidrug Resistance and Ca2+ Concentration in MCF-7/ADR Cells[J].Space Medicine & Medical Engineering,2007,20(5):313-316. |
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| Authors: | CAI Tian-ge CAI Yu YU Shao-lei FENG Xiao-zhen |
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| Abstract: | Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. Results Psoralen from 1 to 20 μmol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration. |
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| Keywords: | psoralen multidrug resistance reverse MCF-7/ADR cells |
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