VEGF硫代反义寡核苷酸抑制U937细胞VEGF的表达

为研究硫代磷酸化修饰的血管内皮生长因子反义脱氧寡核苷酸 (VEGF ASODN)对急性单核细胞白血病细胞系U937VEGF表达的影响 ,将终浓度分别为 10 ,2 0 ,30 μmol/L的VEGF ASODN和错义序列与U937细胞分别孵育 2 4 ,4 8,72小时 ,采用RT PCR检测VEGFmRNA的表达 ,Westernblot检测VEGF蛋白的表达。结果显示 ,VEGF ASODN对U937细胞的VEGF的表达有明显的抑制作用 ,与错义序列组和空白对照组相比有显著差异 (P<0 .0 5 ) ;错义序列组与空白对照组VEGF的表达无显著差异 (P >0 .0 5 )。结论 :VEGFASODN可下调白血病细胞株U937的VEGFmRNA和蛋白的表达水平。

Inhibiting Effect of Vascular Endothelial Growth Factor (VEGF) Antisense Oligodeoxynucleotides on VEGF Expression in U937 Cell

To study the effect of VEGF fully phosporothioated antisense oligodeoxynucleotide (VEGF-ASODN) on VEGF expression in acute monocyte leukemic cell line U937 in vitro, U937 cells were incubated with VEGF-ASODN at concentrations of 10, 20 and 30 micro mol/L or scrambled sequence as compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR. The expression of VEGF protein was measured by Western blot. The result showed that VEGF-ASODN had obviously inhibitive effect on expression of VEGF in U937 cell, as compared with scrambled sequence and negative control (P < 0.05). Scrambled sequence group had no significant difference compared with negative control group (P > 0.05). It is concluded that the expressions of VEGF mRNA and protein in leukemic cell line U937 are down-regulated by VEGF-ASODN.