| 乙酰螺旋霉素组分分析中色谱柱的理性选择方法探讨(英文) |
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| 引用本文： | 王明娟,郑超,胡昌勤,金少鸿,Hoogmartens Jos,VanSchepdael Ann,Adams Erwin.乙酰螺旋霉素组分分析中色谱柱的理性选择方法探讨(英文)[J].中国药学,2011,20(6):572. |
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| 作者姓名： | 王明娟 郑超 胡昌勤 金少鸿 Hoogmartens Jos VanSchepdael Ann Adams Erwin |
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| 作者单位： | 中国食品药品检定研究院;鲁汶大学药物分析实验室;重庆大学生物工程学院; |
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| 摘 要： | 乙酰螺旋霉素为碱性的多组分抗生素,是以螺旋霉素为起始原料的半合成抗生素。乙酰螺旋霉素原料药中除了单-乙酰螺旋霉素Ⅱ和Ⅲ、双-乙酰螺旋霉素Ⅱ和Ⅲ四种主成分外,还能检出70多种小组分,这些小组分的结构与主成分相似或为主成分的同分异构体,故将乙酰螺旋霉素的各组分基线分离很具挑战性;在实际工作中还发现,采用不同品牌的C_(18)柱所得的乙酰螺旋霉素组分测定结果有时差异较大,有必要采用科学的方式理性挑选适宜的色谱柱进行其组分测定。本文利用国际上较有影响力的鲁汶大学所建的色谱柱分类系统数据库,以文献报道的CAPCELL PAK MG C_(18)柱(250 mm×4.6 mm,5μm)作为参比柱,选择了3组选择性不同的色谱柱,并按照中国药典2010版乙酰螺旋霉素各论项下的方法考察了这3组色谱柱的实际分离情况。与参比柱的结果相比,F<2组的分离效果最好,F>6组的分离效果最差,而26组色谱柱(F≥9.9)的绝对差异则超过2.5%,甚至高达8.2%,...
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| 关 键 词： | 乙酰螺旋霉素 半合成抗生素 色谱柱选择 色谱柱分类系统 |
Facilitated column selection in the separation of acetylspiramycin components using a simple column classification system |
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| Ming-Juan Wang,Chao Zheng,Chang-Qin Hu,Shao-Hong Jin,Jos Hoogmartens,Ann Van Schepdael,Erwin Adams.Facilitated column selection in the separation of acetylspiramycin components using a simple column classification system[J].Journal of Chinese Pharmaceutical Sciences,2011,20(6):572. |
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| Authors: | Ming-Juan Wang Chao Zheng Chang-Qin Hu Shao-Hong Jin Jos Hoogmartens Ann Van Schepdael Erwin Adams |
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| Institution: | Ming-Juan Wang~(1,2),Chao Zheng~(2,3),Chang-Qin Hu~(1*),Shao-Hong Jin~1,Jos Hoogmartens~2,Ann Van Schepdael~2,Erwin Adams~2 1.Department of Antibiotics,National Institute for Food and Drug Control,Beijing 100050,China 2.Katholieke Universiteit Leuven,Faculteit Farmaceutische Wetenschappen,Laboratorium voor Farmaceutische Analyse,O&N2,PB 923,Herestraat 49,B-3000 Leuven,Belgium 3.Bioengineering College,Chongqing University,Chongqing 400030,China |
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| Abstract: | Acetylspiramycin(ASPM),a 16-membered basic macrolide antibiotic,is the acetylated derivative of spiramycin.In addition to the four main components,more than seventy minor components could be present in ASPM.Thus,it is a challenge to obtain a baseline separation of ASPM components.Meanwhile,in some cases it was found that the results obtained by different brands of C_(18) columns were significantly different,indicating the necessity of a rational column selection for the separation of ASPM components.In this... |
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| Keywords: | Acetylspiramycin Semi-synthetic antibiotic Column selection Column characterization system |
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