| RP-HPLC-UV法测定大鼠血浆中西瑞香素浓度(英文) |
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| 引用本文： | 孙立新,陈扬,杨雯雯,郭君阳,赵挺,佟立今.RP-HPLC-UV法测定大鼠血浆中西瑞香素浓度(英文)[J].中国药学,2011,20(5):493. |
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| 作者姓名： | 孙立新 陈扬 杨雯雯 郭君阳 赵挺 佟立今 |
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| 作者单位： | 沈阳药科大学药学院;辽宁医药工业研究院; |
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| 基金项目： | National Key Scientific Project for New Drug Discovery and Development(Grant No.2009ZX09301-012) |
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| 摘 要： | 建立了测定大鼠血浆中西瑞香素浓度的高效液相色谱法,并对其进行方法学确证。以蛋白沉淀法和液液萃取法对大鼠血浆中西瑞香素进行提取。采用Diamonsil C_(18)柱(200mm×4.6mm,5μm),流动相为甲醇-20mmol/L醋酸铵(醋酸调pH至3.2,42:58,v/v),流速1.0mL/min。检测波长345nm,柱温40℃。西瑞香素在0.020-2.00μg/mL浓度范围内线性关系良好(r>0.9900),定量下限0.020μg/mL。日内和日间精密度(RSD)为5.0%-10.6%,准确度(RE)为±(1.2%-2.5%)。所建立的方法成功应用于腹腔注射西瑞香素在大鼠体内的药代动力学研究。
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| 关 键 词： | 高效液相色谱 药代动力学 西瑞香素 大鼠血浆 |
Development and validation of an RP-HPLC-UV method for the determination of daphnoretin in rat plasma |
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| Li-Xin Sun,Yang Chen,Wen-Wen Yang,Jun-Yang Guo,Ting Zhao,Li-Jin Tong .School of Pharmacy,Shenyang Pharmaceutical University,Shenyang ,China .Liaoning Institute of Pharmaceutical Industry,Shenyang ,China.Development and validation of an RP-HPLC-UV method for the determination of daphnoretin in rat plasma[J].Journal of Chinese Pharmaceutical Sciences,2011,20(5):493. |
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| Authors: | Li-Xin Sun Yang Chen Wen-Wen Yang Jun-Yang Guo Ting Zhao Li-Jin Tong School of Pharmacy Shenyang Pharmaceutical University Shenyang China Liaoning Institute of Pharmaceutical Industry Shenyang China |
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| Institution: | Li-Xin Sun~(1*),Yang Chen~1,Wen-Wen Yang~1,Jun-Yang Guo~1,Ting Zhao~2,Li-Jin Tong~1 1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China 2.Liaoning Institute of Pharmaceutical Industry,Shenyang 110015,China |
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| Abstract: | |
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| Keywords: | HPLC Pharmacokinetic study Daphnoretin Rat plasma |
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