| 黑米花色苷对白血病细胞株HL-60及正常淋巴细胞活性氧及线粒体膜电位的影响 |
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| 引用本文： | 常徽,糜漫天,顾艳艳,袁家林,凌文华,林辉. 黑米花色苷对白血病细胞株HL-60及正常淋巴细胞活性氧及线粒体膜电位的影响[J]. 第三军医大学学报, 2008, 30(7): 585-587 |
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| 作者姓名： | 常徽 糜漫天 顾艳艳 袁家林 凌文华 林辉 |
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| 作者单位： | 1. 第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室,重庆,400038
2. 中山大学公共卫生学院营养系,广州,510080 |
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| 摘 要： | 目的 观察黑米花色苷对白血病HL-60细胞和正常淋巴细胞活性氧(reactive oxygen species,ROS)水平及线粒体膜电位的影响.方法 以2,7-二氯荧光素(2,7-dichloro fluoreseeineiactate,DCFH-DA)为细胞内活性氧探针,以罗丹明-123为反映线粒体膜电位荧光探针,流式细胞仪和激光共聚焦扫描显微镜检测分析黑米花色苷作用下HL-60细胞及正常淋巴细胞活性氧水平及线粒体膜电位的变化.结果 黑米花色苷处理正常淋巴细胞2、24 h其ROS水平未见显著变化(P＞0.05);黑米花色苷处理HL-60细胞,ROS水平首先显著升高(P＜0.01),随后逐渐降低,并在12 h显著低于对照纽(P＜0.01).黑米花色苷处理正常淋巴细胞24 h线粒体膜电位无显著变化(P＞0.05),处理HL-60细胞24 h线粒体膜电位显著降低(P＜0.01).结论 黑米花色苷能够显著影响HL-60细胞活性氧水平,并显著降低HL-60细胞线粒体膜电位,而对正常淋巴细胞活性氧及线粒体膜电位无显著影响.
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| 关 键 词： | 黑米花色苷 HL-60细胞 正常淋巴细胞 活性氧 线粒体膜电位 |
| 文章编号： | 1000-5404(2008)07-0585-03 |
| 修稿时间： | 2007-07-19 |
Effects of anthocyanin-rich extract from black rice on ROS and mitochondrial membrane potential of leukemia HL-60 cells and human lymphocytes |
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| CHANG Hui,MI Man-tian,GU Yan-yan,YUAN Jia-lin,LING Wen-hua,LIN Hui. Effects of anthocyanin-rich extract from black rice on ROS and mitochondrial membrane potential of leukemia HL-60 cells and human lymphocytes[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(7): 585-587 |
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| Authors: | CHANG Hui MI Man-tian GU Yan-yan YUAN Jia-lin LING Wen-hua LIN Hui |
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| Affiliation: | 1Department of Nutrition and Food Hygiene;College of Preventive Medicine;Third Military Medical University;Chongqing 400038;2Department of Nutrition;Public Health School;Sun Yet-sen University;Guangzhou 510080;China |
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| Abstract: | Objective To observe the effects of anthocyanin-rich extract from black rice on reactive oxygen species (ROS) and mitochondrial membrane potential of leukemia HL-60 cells and human lymphocytes. Methods Using a ROS probe (2, 7-dichloro fluorescein diactate, DCFH-DA) and rhodamine-123, the levels of ROS and mitochondrial membrane potential of HL-60 cells and human lymphocytes were determined by flow cytometry and laser confocal scanning microscopy. Results The extract had no significant effects on ROS level o... |
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| Keywords: | anthocyanin-rich extract from black rice HL-60 cell line human lymphocytes reactive oxygen species mitochondria membrane potential |
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