黄芪、当归及其组方促血管内皮细胞增殖作用的研究

目的：探讨黄芪、当归及其组方对人脐静脉内皮细胞增殖和细胞周期的影响。方法：以离体培养的人脐静脉内皮细胞(human umbilical vein endothelial cells,，HUVEC)为模型，采用MTT法测定细胞增殖、流式细胞仪分析其细胞周期、SABC法检测血管内皮生长因子(vascular endothelial growth factor，VEGF)的表达，对黄芪、当归及其组方的促HUVEC增殖作用进行了观察研究。结果：黄芪、当归单用或合用均可促进内皮细胞生长，增加细胞周期中S期细胞的数目，尤以复方作用明显(P<0．05或P<0．001)。同时，各给药组均能上调VEGF的表达，而对照组VEGF仅有微弱表达(P<0．05或P<0．01)。结论：黄芪、当归有促进血管内皮细胞增殖及DNA合成的作用，两药配伍应用时具有协同效应，提示在缺血心肌新生血管的发生和发展中可能具有一定作用。

Study on Effects of Astragalus, Angelica and Their Combination on Vascular Endothelial Cell Proliferation in vitro

Objective: To study the effects of Astragalus membranaceus (AM), Angelica sinensis (AS) and their combination on human umbilical vein endothelial cell (HUVEC) proliferation and cells cycle. Methods: The effects were observed and studied by means of taking the cultured HUVECs as model to determine the cell proliferation with MTT method, cell cycle was analyzed with cytometry, and vascular endothelial growth factor (VEGF) expression with SABC method. The regulatory effects of AM, AS and their combination on the HUVEC proliferation promoting were observed and studied. Results: AM and AS, used singly or in combination, could promote the growth of endothelial cells, increase the cell population in S phase, the effects showed more significant when used in combination (P<0.05 or P<0.001). Meanwhile, VEGF expression in all the medicated group was up-regulated, but in the PBS control group, it showed only weak expression (P<0.05 or P<0.01). Conclusion: AM and AS have effect in promoting vascular endothelial cell proliferation and DNA synthesis, and showed synergistic effect when they were used in combination, suggesting that these two Chinese herbs could have certain effect on the genesis and development of neogenetic vascularization in ischemic myocardium.