人肺癌染色体端粒联合的比较观察

目的 探讨人肺癌细胞染色体端粒联合异常的规律,方法 应用染色体制备法检测人肺腺癌细胞株Ａ５４９细胞、１５例肺癌患者外周血淋巴细胞的端粒联合率,并以１５例良性疾病和正常人作对照。结果 肺癌组和非癌对照组的端粒联合率存在显著差异（Ｐ〈０．００５）,人肺腺癌细胞株Ａ５４９中端粒联合率显著高于肺严组和非癌对照组（Ｐ〈０．００５）;肺癌组细胞染色体中Ｂ、Ｃ、Ｄ组的端粒联合率明显高于非癌对照组（Ｂ组Ｐ〈０．０

Comparison and observation on chromosome telomeric associations in human lung cancer.

Objective To explore the regularity of abnormal telomeric associations in cellular chromosome of human lung cancer. Methods The rate of telomeric associations was detected in human lung adenocarcinoma cell line A549, peripheral blood lymphocytes in 15 patients with lung cancer, and in patients with non cancerous diseases and normal adults as control by chromosome preparation assay. Results A highly significant difference in the rate of telomeric associations was found between lung cancer group and control group (P<0.005). Human lung adenocarcinoma cell line A549 had the highest rate of telomeric associations in all groups (P<0.005). Moreover, there was significant difference in the rate of telomeric associations in idiogram B, idiogram C and idiogram D between lung cancer group and control group (idiogram B,P<0.05; idiogram C, idiogram D, P<0.005). Conclusion Compared with control group, human lung adenocarcinoma cell line A549 and lung cancer chromosome has higher telomeric associations and most of them occured in idiogram B, idiogram C and idiogram D. It will provide worthwhile data of cell genetics for further research work on early diagnosis and prognosis in human lung cancer.