The effect of diclofenac on matrix metalloproteinase levels in the rotator cuff |
| |
Authors: | Haluk Çabuk Arzu Avci Hüseyin Durmaz Fatmagül Kuşku Çabuk Fatih Ertem İ. Muhittin Şener |
| |
Affiliation: | 1. Department of Orthopedics and Traumatology, Okmeydan? and Research Hospital, ?i?li, Turkey 2. Department of Medical Pathology, ?zmir Katip ?elebi University Atatürk Training and Research Hospital, ?zmir, Turkey 3. Department of Orthopedics and Traumatology, ?zmir Katip ?elebi University Atatürk Training and Research Hospital, ?zmir, Turkey 4. Department of Medical Pathology, ?stanbul Bilim University, ?stanbul, Turkey 5. Department of Biomechanics, Dokuz Eylül University, Health Science Institute, ?zmir, Turkey
|
| |
Abstract: | Introduction Matrix metalloproteinases (MMPs) are involved in physiological events such as restructuring of the tissue, morphogenesis, wound healing and normal developmental process. Use of diclofenac sodium following rotator cuff repair can disrupt healing of tendon through acting on MMPs. Materials and methods Supraspinatus tendons of rats (n = 84) were detached from their insertion on humerus, and repaired to anatomic footprint. Rats were divided into study group (n = 42) and control group (n = 42). Study group received a dose of 1 mg/kg daily diclofenac sodium subcutaneously. The rats were killed at weeks 1, 3 and 6, and seven rats from each groups were included in biomechanical and immunohistological examinations. Immunohistological staining of MMP-2, MMP-3 and MMP13 were used. Results Maximum load was reduced in the study group at the end of week 1 (8.76 vs. 5.28 N) (p = 0.01). MMP-3 level was statistically significantly lower in the study group at the end of week 1. MMP-13 level and stiffness decreased towards week 6 in the study group while in the control group the level of MMP-2 decreased towards week 6. Conclusion Diclofenac has an impact on the levels of MMP-2, MMP-3 and MMP-13, which are needed for normal healing process, and it can also lead to disruption of tendon healing. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|