尼美舒利抑制结肠癌侵袭和转移的作用机制研究

目的观察选择性环氧合酶-2(COX-2)抑制剂尼美舒利对结肠癌细胞Caco-2同质性黏附能力,E-钙黏蛋白和CD44v6表达的影响.探讨其在抑制肿瘤侵袭和转移中的作用机制.方法培养结肠癌细胞细胞株Caco-2,用不同浓度的尼美舒利进行干预,观察细胞同质性黏附能力的变化.用Western blot检测E-钙黏蛋白表达的变化,用免疫细胞化学法检测CD44v6的变化.结果尼美舒利能增加Caco-2细胞的同质性黏附能力,增加E-钙黏蛋的表达,并呈剂量依赖性.结论尼美舒利可以通过增强结肠癌细胞的同质性黏附能力,增加E-钙黏蛋白的表达,减少CD44v6的表达来抑制肿瘤细胞的侵袭和转移.

The Study on Mechanism of Nimesulide in Inhibiting the Invasion and Metastasis in Colon Cancer

Objective To characterize the effects of nimesulide , a selective cyclooxyegenase-2 inhibitor ,on homotypic adhesion and the expression of E-cadherin and CD44v6 . To iwestigate the mechanism of inhibitory tumor invasion and metastasis in colon cancer . Methods The human colon cancer cells Caco-2 were cultured . After incubated with nimesulide in different concentrations ,the function of homotypic adhesion was measured , The expression of E-cadherin was detected by Western blotting . The expression of CD44v6 was detected by immunocytochemistry method . Results Nimesulide enhanced the function of homotypic adhesion . Nimesulide increased the expression of E-cadherin . And nimesulide decreased the expression of CD44v6. Conclusion Nimesulide can inhibit the invasion and metastasis of colon cancer by enhancing the function of homotypic adhesion ,increasing the expression E-cadherin and decreasing the expression of CD44v6.