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| 正交设计-重复实验法优选丹参骨宝酸提取工艺的最佳条件 | |
| 引用本文： | 刘力名,吕应年,崔燎,吴铁.正交设计-重复实验法优选丹参骨宝酸提取工艺的最佳条件[J].时珍国医国药,2010,21(2). |
| 作者姓名： | 刘力名 吕应年 崔燎 吴铁 |
| 作者单位： | 1. 广东医学院,广东,湛江,524023;广东天然药物研究开发实验室,广东,湛江,524023 2. 广东天然药物研究开发实验室,广东,湛江,524023 3. 广东医学院,广东,湛江,524023 |
| 摘 要： | 目的 采用正交实验考察丹参骨宝的酸提取液制备工艺的最佳条件.方法 以丹参素钠与丹酚酸B含量之比作为评价指标,对溶剂(A)、溶剂量(B)、提取时间(C)、提取次数(D)等4个因素的3个水平进行L_9(3~4)正交实验.结果 直观分析和方差分析显示酸提取工艺因素的影响大小依次是:溶剂>溶剂量>提取时间>提取次数,最佳提取工艺条件为A_3B_2C_1D_3,即5 g丹参粗粉加4%盐酸50 ml回流提取0.5 h,共提取3次.结论 通过正交实验筛选出了丹参骨宝最佳的酸提条件. |
| 关 键 词： | 丹参骨宝 正交设计 高效液相色谱法 |
Study on Optimization of Experimental Conditions for Acid Extraction of Danshen Gubao by Orthogonal Design-Duplicate Test |
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| LIU Li-ming,LV Ying-nian,CUI Liao,WU Tie.Study on Optimization of Experimental Conditions for Acid Extraction of Danshen Gubao by Orthogonal Design-Duplicate Test[J].Lishizhen Medicine and Materia Medica Research,2010,21(2). | |
| Authors: | LIU Li-ming LV Ying-nian CUI Liao WU Tie |
| Institution: | LIU Li-ming1,2,LV Ying-nian2,CUI Liao1,WU Tie1(1.Department of Pharmacology of Guangdong Medical College,Zhanjiang,Guangdong 524023,China,2.Guangdong Key Laboratory for R&D of Natural Drugs,Zhanjiang Guangdong 524023,China) |
| Abstract: | Objective To obtain the optimization condition for preparation of Danshen Gubao acid extraction solution using orthogonal design.Methods Using the ratio of contents of Danshensu-Na and Salvianolic Acid B as an evaluation standard,the L9(34) experiment was carried out in consideration of 3 levels of 4 factors including solvent(A),dosage of solvent(B),extraction time(C,h),number of extraction times(D).Results The optimized extraction conditions calculated by the intuitive analysis and ANOVA was A3B1C1D3,5 g S... |
| Keywords: | Danshen Gubao Orthogonal design HPLC |
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