苯那普利对动脉粥样硬化形成及循环内皮细胞与假血管血友病因子的影响作用

观察苯那普利对高胆固醇饲喂的家兔动脉粥样硬化形成及其对循环内皮细胞 (CEC)、假血管血友病因子(vWF)的影响。 2 4只新西兰雄性大白兔随机分成 3组 ,对照组饲喂普通饲料 ,动脉粥样硬化组饲喂 1 %胆固醇饲料 ,苯那普利组饲喂 1 %胆固醇及苯那普利5mg/d。实验前、实验后 5周、实验后 1 0周分别抽血测血脂、vWF、CEC计数。第 1 0周末处死大白兔 ,计算主动脉斑块面积。结果显示 ,苯那普利治疗组与动脉粥样硬化组血脂水平无明显变化 ,但动脉粥样硬化斑块面积却明显减少(P <0 0 1 ) ,其血浆vWF浓度亦明显降低(P <0 0 1 )。提示苯那普利具有抗动脉粥样硬化作用 ,其机制可能与保护内皮功能有关

STUDY ON THE EFFECT OF BENAPRIL ON ATHEROGENESIS, CIRCULATING ENDOTHELIAL CELLS AND FACTOR vWF

To examine the effect of benapril on atherogenesis, circulating endothelial cells (CEC), and factor vWF. Twenty four healthy male New Zealand white rabbits were randomly divided into 3 groups. In group C (control group) the rabbits were fed normal rabbit diet for ten weeks, in group AS 1% cholesterol diet was fed, and in group B both 1% cholesterol diet and benapril 5mg/day were fed. Fasting blood samples were collected for the determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL c), low density lipoprotein cholesterol (LDL c), and triglyceride (TG) on day 0, 5 and 10 weeks after feeding. The levels of plasma CEC were measured by morphometrical count and the levels of plasma vWF were determined by ELISA. After the animals were sacrificed, aortas were obtained, and they were stained with oil red O. The tissue was sectioned for histological examination. Planimetry was performed with a computer system. Endothelial cells were identified by the presence of factor VIII related antigen, which was the specific marker for endothelial cells. The results showed that the levels of serum Tc, TG, and LDL c were increased progressively in group B and group AS, but the levels of vWF in group AS were significantly higher than that in group B at the 5 th and 10 th weeks respectively ( P <0 01), whereas there was no significant difference between the number of CEC in all groups. The results suggested that benapril was able to inhibit the further development of atherosclerosis by protecting the endothelial function.