|
|||||
|
|
Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis |
|
| Authors: | Bashir Salim Mohammed Ahmed Bakheit Chihiro Sugimoto |
| Affiliation: | 1. Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, 13314, Khartoum North, Sudan 2. School of Biology, Centre for Genetics and Genomics, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK 3. Veterinary Infection Biology and Immunology, Research Center Borstel, 23845, Borstel, Germany 4. Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan |
| Abstract: | The application of high-resolution melting (HRM) analysis in the differentiation between Theileria equi and Babesia caballi was evaluated using control samples from the United States Department of Agriculture and field samples collected from horses in Sudan and China. A region of the 18S rRNA gene, with four known nucleotide differences between the two parasites, was selected for primer design. HRM analysis successfully allowed the detection and differentiation of T. equi and B. caballi without the necessity of performing time-consuming and expensive post-PCR procedures such as sequencing or restriction digestion. Our results suggest that HRM could be an ideal method for rapid genotyping, which is required to determine a drug of choice or to administer an appropriate vaccine during an outbreak. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |