| 组织工程化人工神经构建的方法研究 |
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| 引用本文： | 程飚,陈峥嵘. 组织工程化人工神经构建的方法研究[J]. 中国矫形外科杂志, 2002, 9(5): 456-459 |
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| 作者姓名： | 程飚 陈峥嵘 |
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| 作者单位： | 上海复旦大学附属中山医院骨科,上海枫林路180号,200032 |
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| 基金项目： | 上海市科学技术发展基金 (项目编号 0 1 4 0 1 90 2 8)，国家863启动金 (EXF0 0 0 31 8) |
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| 摘 要： | 目的：探索用组织工程方法构建人工神经的可行性。方法：用鼠尾胶和层粘蛋白共同修饰的polyglytin 910纤维以及生物膜作为支架与成年兔雪旺细胞培养2周。观察细胞生长迁移情况。结果：雪旺细胞能在共聚物纤维上迁移包裹，均匀分布于支架之间及吸附在支架表面。雪旺细胞在生物膜上能吸附生长。结论：成年雪旺细胞可以在修饰后的polylytin910纤维和生物膜上得到扩增，二者共同形态的三维立体结构具有人工神经的基本特性。
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| 关 键 词： | 雪旺细胞 体外培养 生物材料 组织工程 |
| 文章编号： | 1005-8478(2002)05-0456-04 |
| 修稿时间： | 2001-06-08 |
Study of Fabricating Tissue Engineering Method for Artificial Nerve |
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| CHENG Biao,CHEN Zheng rong. Study of Fabricating Tissue Engineering Method for Artificial Nerve[J]. The Orthopedic Journal of China, 2002, 9(5): 456-459 |
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| Authors: | CHENG Biao CHEN Zheng rong |
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| Affiliation: | CHENG Biao,CHEN Zheng rong.Department of Orthopedics,Zhongshan Hospital,Medical Center of Fudan University,Shanghai 200032 |
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| Abstract: | Objective: To investigate the possibility of fabricating artificial nerves with tissue engineering methods.Method:The Schwann cells were cultured when they were seeded on polyglactin 910 scaffolds and biomembrane coating with rat tail glue and laminin together and respectively for 2 weeks.The observation of the scaffolds'adsorptivity to Schwann cells,the growth and migration of the Schwann cells were made.Result:The Schwann cells could migrate on the fibre and wrapped fibre up with circle.Schwann cell were well distributed.Schwann cell on biomembrane could also grow well.Conclusion:Adult SC can be expanded on the coated fibre and the biomembrane.Three dimension scaffolds with Schwann cell had the basic characteristics of the artificial nerve. |
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| Keywords: | Schwann cell Cell cultutre in vitro Biomaterials Tissue engineering |
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