| 蛋白保护剂对蛇毒抗肿瘤活性物质ACTX-6的保护作用 |
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| 引用本文： | 章良,韦利军,黄凤杰,吴梧桐.蛋白保护剂对蛇毒抗肿瘤活性物质ACTX-6的保护作用[J].中国药科大学学报,2005,36(2):183-186. |
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| 作者姓名： | 章良 韦利军 黄凤杰 吴梧桐 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009 |
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| 摘 要： | 目的：找到一种冻干赋形剂，能有效用于蛇毒中抗肿瘤活性物质ACTX-6的保护作用。方法：采用正交实验设计原理和方法，以海藻糖、丙氨酸、NaCl作为冻干赋形剂的组成，筛选了它们的配比，考察样品冻干后的电泳区带和细胞毒性。结果：所找到的赋型剂的配方不仅可以使ACTX-6在冻干后电泳区带和细胞毒性都不改变，并且能够明显改善样品蛋白对动物的毒性，腹腔注射的LD50在加了保护剂后得到提高，静脉给药时局部组织坏死现象得到改善。
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| 关 键 词： | 冻干保护剂 正交试验 体外细胞毒性 半数致死率 |
| 文章编号： | 1000-5048(2005)02-0183-04 |
| 修稿时间： | 2004年7月13日 |
Effect of a Proposed Lyoprotectant on the Cytotoxin ACTX-6 Purified from Snake Venom |
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| ZHANG Liang,WU Wu-Tong.Effect of a Proposed Lyoprotectant on the Cytotoxin ACTX-6 Purified from Snake Venom[J].Journal of China Pharmaceutical University,2005,36(2):183-186. |
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| Authors: | ZHANG Liang WU Wu-Tong |
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| Abstract: | AIM:To screen a lyoprotectant which coul d stabilize the cytotoxin ACTX-6 purified from Agkistrodon acutus snake venom. METHOD:Based on the electrophoretogram and cytotoxic activity of freeze-dried powder of ACTX-6 in the presence of various proposed protectants , orthogonal experiment design was used to screen one suitable protectant formul a including trehalose,alanine and NaCl.RESULT:Formula as an effec tive protectant was screened out, which could not only ensure no changes in electrop horetogram and cytotoxic activity in vitro of the freeze-dried ACTX-6 b ut also increase the magnitude of LD 50 of ACTX-6.In addition,if the lyop rotectant was added, LD 50 of ACTX-6 i.p. was increased, and the side effe ct was decreased when dosing i.v.. |
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| Keywords: | Lyoprotectant Orthogonal experiment de sign Cytotoxic activity in vitro LD 50 |
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