Neurotoxic effects of lambda‐cyhalothrin modulated by piperonyl butoxide in the brain of Oreochromis niloticus

The objective of this research was to investigate the neurotoxic effects of pyrethroid pesticide lambda‐cyhalothrin by the modulation of cytochrome P450 with piperonyl butoxide in the brain of juvenile Oreochromis niloticus. The fish were exposed to 0.48 μg L^?1 (1/6 of the 96‐h LC₅₀) lambda‐cyhalothrin and 10 μg L^?1 piperonyl butoxide for 96 h and 15 days. tGSH, GSSG, TBARS contents, GPx, GR, GST, and AChE enzymes activities were determined by spectrophotometrical methods and Hsp70 content was analyzed by ELISA technique. Lambda‐cyhalothrin had no significant effect on the components of GSH redox system, lipid peroxidation and Hsp70 levels but inhibited AChE activity. In the presence of piperonyl butoxide, lambda‐cyhalothrin caused increases in tGSH, GSSG, TBARS and Hsp70 contents, GST activity, and decrease in AChE activity. Present results showed that in the presence of piperonyl butoxide, lambda‐cyhalothrin caused neurotoxic effects by increasing oxidative stress. Adaptation to its oxidative stress effects may be supplied by GSH‐related antioxidant system. Piperonyl butoxide revealed neurotoxic effect of lambda‐cyhalothrin. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1275–1282, 2014.