Regenerative effect of adipose tissue‐derived stem cells transplantation using nerve conduit therapy on sciatic nerve injury in rats |
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Authors: | Bai‐Shuan Liu Yi‐Chin Yang Chiung‐Chyi Shen |
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Institution: | 1. Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, , Taichung, Taiwan, Republic of China;2. Department of Neurosurgery, Taichung Veterans General Hospital, , Taichung, Taiwan, Republic of China;3. Faculty of Medicine, School of Medicine, National Yang‐Ming University, , Taipei, Taiwan, Republic of China |
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Abstract: | This study proposed a biodegradable GGT nerve conduit containing genipin crosslinked gelatin annexed with tricalcium phosphate (TCP) ceramic particles for the regeneration of peripheral nerves. Cytotoxicity tests revealed that GGT‐extracts were non‐toxic and promoted proliferation and neuronal differentiation in the induction of stem cells (i‐ASCs) derived from adipose tissue. Furthermore, the study confirmed the effectiveness of a GGT/i‐ASCs nerve conduit as a guidance channel in the repair of a 10‐mm gap in the sciatic nerve of rats. At eight weeks post‐implantation, walking track analysis showed a significantly higher sciatic function index (SFI) (P < 0.05) in the GGT/i‐ASC group than in the autograft group. Furthermore, the mean recovery index of compound muscle action potential (CMAP) differed significantly between GGT/i‐ASCs and autograft groups (P < 0.05), both of which were significantly superior to the GGT group (P < 0.05). No severe inflammatory reaction in the peripheral nerve tissue at the site of implantation was observed in either group. Histological observation and immunohistochemistry revealed that the morphology and distribution patterns of nerve fibers in the GGT/i‐ASCs nerve conduits were similar to those of the autografts. These promising results achieved through a combination of regenerative cells and GGT nerve conduits suggest the potential value in the future development of clinical applications for the treatment of peripheral nerve injury. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | biodegradable nerve conduit cytotoxicity adipose tissue‐derived stem cells tissue engineering sciatic function index peripheral nerve regeneration |
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