| 逆向斑点杂交检测乙型肝炎病毒基因型及YMDD变异 |
| |
| 引用本文： | 黄燕萍,兰林,李俊刚,丁世涛,范燚,王宇明.逆向斑点杂交检测乙型肝炎病毒基因型及YMDD变异[J].中华检验医学杂志,2006,29(2):140-142. |
| |
| 作者姓名： | 黄燕萍 兰林 李俊刚 丁世涛 范燚 王宇明 |
| |
| 作者单位： | 400038,重庆,第三军医大学西南医院全军感染病研究所 |
| |
| 摘 要： | 目的 应用逆向斑点杂交技术检测乙型肝炎病毒（HBV）基因型及YMDD变异。方法 合成有生物素标记的引物，PCR扩增两种DNA片段，与尼龙膜上的探针同时杂交，BCIP／NBT显色。结果 检测21例拉米夫啶治疗中的慢性乙型肝炎患者，11例HBV为基因型B，10例为基因型C；YMDD基序有47．6％（10／21）为YMDD，28．6％（6／21）为YVDD，23．8％（5／21）为YIDD。结论 在同一张尼龙膜上检测HBV基因型及YMDD变异，经济实惠、准确快捷，且易于开展。
|
| 关 键 词： | 印迹法 DNA 肝炎病毒 乙型 基因型 |
| 收稿时间： | 2005-04-06 |
| 修稿时间： | 2005年4月6日 |
Study of HBV genotypes and YMDD mutations by reverse dot blot |
| |
| HUANG Yan-ping,LAN Lin,LI Jun-gang,DING Shi-tao,FAN Yi,WANG Yu-ming.Study of HBV genotypes and YMDD mutations by reverse dot blot[J].Chinese Journal of Laboratory Medicine,2006,29(2):140-142. |
| |
| Authors: | HUANG Yan-ping LAN Lin LI Jun-gang DING Shi-tao FAN Yi WANG Yu-ming |
| |
| Institution: | Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China |
| |
| Abstract: | Objective HBV genotypes and YMDD mutations were detected by reverse dot blot.Method Two PCR-amplified fragments generated by biotin labeled primers were complementary to the oligonucleotides that were fixed on the nylon membrane and then detected the signals by BCIP/NBT.Result In 21 Lamivudine therapy patients, there were 11genotype B and 10 genotype C.The YMDD motif was 47.6%(10/21)YMDD, 28.6%(6/21)YVDD, and 23.8%(5/21)YIDD.Conclusion Detection of HBV genotypes and YMDD mutations together were economical, exact, rapid and easy. |
| |
| Keywords: | Blotting Southern Hepatitis B virus Genotype |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
