血小板活化因子与肿瘤坏死因子和白介素8在大鼠脓毒血症中的作用

目的 探讨血小板活化因子（PAF）及其拮抗剂与肿瘤坏死因子（TNF）和白介素8（IL－8）在大鼠脓毒血症中的作用。方法 应用大鼠盲肠结扎加穿孔模型，在不同时相点观察大鼠死亡率并检测其血PAF，血清TNF及IL－8的水平。结果 （1）严重腹腔感染后，血PAF、血清TNF及IL－8水平明显升高，且前者与后两者呈显著正相关。（2）应用PAF拮抗剂WEB2170治疗后，血PAF、血清TNF及IL－8水平可显著降低。（3）WEB2170能明显降低严重腹腔感染大鼠的死亡率。结论 脓毒血症早期应用PAF拮抗剂可阻挡PAF与TNF和IL－8的网络联系，对防治多器官功能衰竭具有重要意义。

Role of platelet activating-factor (PAF) ,tumor necrosis factor(TNF), interleuking8(IL-8)on septic rats

Objective To investigate the role of PAF, TNF, IL 8 on septic rats. Methods Made use of the model of cecum ligated and perforated, observed the mortality rate of septic rats and examined their level of blood PAF, serum TNF,IL 8 at several plastic points, analysizd the relationship between level of blood PAF and serum TNF,IL 8. Results (1)The level of blood PAF, and serum TNF,IL 8 elevated significantly. The blood PAF was positively related with serum TNF,IL 8. (2)Treated with PAF antagonist WEB2170 could reduced the level of blood PAF, serum TNF,IL 8.(3)The mortality rate of the severe infectious rats could be reduced by WEB2170. Conclusion The PAF antagonist can block the network relationship between PAF and TNF,IL 8 and has an important role on preventing MOF.