1例肺腺癌组织的原代培养及异种移植检测

目的选取1例云南宣威女性肺腺癌组织进行原代培养,观察其生长增殖及异种动物接种成瘤情况,建立理想的实验模型.方法以手术切除的无菌肺癌组织标本进行原代培养,以有限稀释法获克隆化生长的肺腺癌细胞,通过光镜、生长曲线、异种动物成瘤实验、免疫组化等对其生物学特性进行分析.结果体外培养细胞生长稳定、状态良好,细胞形态学、增殖动力学实验表明该细胞系符合恶性肺腺癌细胞特征;动物接种成瘤率为100%,表明肿瘤对正常组织的侵袭能力,免疫组化结果证实瘤细胞为腺癌细胞,与原患者的病理切片相同.结论通过一系列对该细胞的体外研究证实本细胞株为恶性肺腺癌细胞,是一株具有地区特异性的肺腺癌细胞株,对研究肺癌癌变、侵袭转移、生物学特征以及开发抗肺癌新药等均有十分重要的理论和临床意义.

The Heterogeneity Transplant Detection and Primary Culture of Lung Cancer in 1 Patient

Objective The aim of our present study is to culture a new female lung adenocarcinoma cell line in Xuanwei region of Yunnan province,detect its proliferation dynamics and heterogeneity transplant and provide an ideal experimental model for the study of lung cancer.Methods The cell line was derived from a surgical specimen of lung cancer,and obtained lung adenocarcinoma cell clone by limiting dilution.Its biologic characteristics were explored by light microscope,determination of the doubling time and growth curve,inoculation the cells in mice as well as immunohistochemistry.Results Morphological study,proliferation dynamics and immersed growth demonstrated the cultured cells' malignant characteristics.The cell line produced 100% tumors after axillary transplantation into the mice provided by the Animal Centre of Kunming Medical College.The results of immunohistochemistry indicated the cells' adenocarcinoma characteristics.Conclusions According to a series of analysis of the cell line in vitro,the present study gave an important implication and some theory basis of following research on human lung adenocarcinoma,biological characteristics,cancerization,the mechanism of metastasis,and exploitation for new anticancer medicine et al.