| 人RGS1基因克隆表达及其功能分析 |
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| 引用本文： | 刘昱,焦国慧,张灼寒,曾斌,陈思,张圆,杨荣存. 人RGS1基因克隆表达及其功能分析[J]. 免疫学杂志, 2009, 25(1) |
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| 作者姓名： | 刘昱 焦国慧 张灼寒 曾斌 陈思 张圆 杨荣存 |
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| 作者单位： | 南开大学医学院免疫学教研室,天津,300071 |
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| 基金项目： | 国家自然科学基金，国家科技部基金，国家高技术研究发展计划(863计划)，天津市科委基金 |
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| 摘 要： | 目的 克隆并构建人RGS1表达载体,转染U937细胞和树突细胞,进行功能分析.方法 通过RT-PCR方法克隆人RGS1基因,将其连接到peDNA 3.1/V5表达载体.然后以连接的质粒为基础通过报告基因化学发光及ELISA方法对人RGS1基因的进行初步分析.结果 经扩增及序列测定表明pcDNA-RGS1质粒构建成功,在U937细胞和树突细胞中可以表达.RGS1对U937细胞转录因子NF-kB的活性有明显的抑制作用,同时明显上涮树突细胞细胞凶子IL-6的表达.结论 在peDNA-RGS1质粒成功构建基础上,证明了人RGS1基因具有免疫调节功能,为进一步研究其免疫应答中的调节作用奠定了基础.
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| 关 键 词： | 免疫调节 RGS1基因 树突细胞 |
Cloning,expression,and function of RGS1 |
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| LIU Yu,JIAO Guohui,ZHANG Zhuohan,ZENG Bin,CHEN Si,ZHANG Yuan,YANG Rongcun. Cloning,expression,and function of RGS1[J]. Immunological Journal, 2009, 25(1) |
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| Authors: | LIU Yu JIAO Guohui ZHANG Zhuohan ZENG Bin CHEN Si ZHANG Yuan YANG Rongcun |
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| Affiliation: | LIU Yu,JIAO Guohui,ZHANG Zhuohan,ZENG Bin,CHEN Si,ZHANG Yuan,YANG Rongcun Department of Immunology,School of Medicine,Nankai University,Tianjin 300071,China |
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| Abstract: | Objective To clone and construct the RGS1 expression plasmid and investigate its expression and function following transfection into U937 and dendritic cells.Methods Human RGS1 was cloned to pcDNA3.1/V5 plasmid,and then the functional potential of human RGS1 in regulating immune responses was investigated via the chemiluminescence and ELISA techniques.Results The plasmid was confirmed by PCR amplification and DNA sequencing,while target gene expression was detected in U937 and dendrite cells.RGS1 could inhi... |
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| Keywords: | immune regulation RGS1 dendritic cell |
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