口腔龋坏组织中变异链球菌与乳酸杆菌的作用研究

目的分析人龋坏组织中变异链球菌与乳酸杆菌在不同分组中的培养计数及不同细菌主导的病例数，研究变异链球菌和乳酸杆菌在致龋过程中的协同作用。方法选取甘肃省靖煤公司总医院口腔科110例龋病患者，按照不同龋坏程度、不同年龄、不同龋坏性质分组采集龋坏组织，在选择培养基上培养，计数菌落数量。统计2种细菌在不同分组中的检出率、细菌计数平均数及不同致龋菌病例数，研究二者的致龋作用。结果变异链球菌和乳酸杆菌的细菌计数平均数随着龋坏程度增加而增高（P<0.05），在老年组患者中变异链球菌和乳酸杆菌数量增高（P<0.05），在龋病活动期增高（P<0.05）。随着龋坏程度的增加，变异链球菌和乳酸杆菌共同致龋病例数增高（P<0.05），在老年人龋坏中共同致龋病例数增高（P<0.05），变异链球菌和乳酸杆菌共同致龋作用与龋病的静止与活动无相关性。结论变异链球菌和乳酸杆菌随着龋坏程度的增加，共同致龋作用增强；老年人龋齿中，二者的协同作用占主导作用；在龋病活动期与静止期中，变异链球菌和乳酸杆菌仅存在数量上的区别，二者的单独致龋能力均在活动期增强，但共同致龋作用与龋病的活动与静止无关联。

Research on cooperation between Streptococcus mutatis and Lactobacilli in dental caries lesion

Objective To analyze the count of Streptococcus mutatis(S.mutans)and Lactobacilli(LB)in different groups and the cases in dental caries.To research the synergistic effect of S.mutans and LB in the process of dental caries.Methods 110 cases with dental caries were selected and divided according to the different degree of caries,nature and ages.To culture bacteria in the selective culture medium,then count the number of colonies.The detection rate of two kinds of bacteria in different groups,means of the bacteria count and the cariogenic cases were analyzed.Results The means of the two bacteria count increased along with the degree of caries increased(P< 0.05),and increased in the older group(P<0.05)and the active stage(P<0.05).The cases of two bacteria increased with the degree of caries increased(P<0.05),and increased in the older group(P<0.05).But there were no significant differences in evolution period and arrested caries.Conclusion The pathopoiesis capability of S.mutans and LB enhanced with the extent of caries increased.In the older group,their synergism role play a lead position.In evolution period and arrested caries,S.mutans and LB were difference only in quantity and their solo cariogenic potential all enhanced in active stage,but there were not correlation on pathopoiesis capability and active or stationary phase.