白细胞介素17在大鼠纤维化模型中的作用及意义

目的研究白细胞介素17（IL-17）在大鼠肺纤维化中的表达及意义。方法健康SD大鼠64只随机分为健康对照组（N组）、肺纤维化模型组（B组）及地塞米松治疗组（D组）。分别于实验的第7、14、28天将N组、B组处死，D组于第14、28天处死，检测支气管灌洗液及肺组织中IL-17表达水平；观察其病理学改变并测定肺组织中羟脯氨酸水平。结果（1）B组肺组织病理切片表现为逐渐由肺泡炎至纤维化动态改变过程，肺组织内羟脯氨酸高于N组和D组（P〈O．05）。（2）在B组和D组中，肺组织和支气管灌洗液中的IL-17的表达趋势相同，都于第7天达到峰值，而在N组中IL-17呈低水平表达，B组和D组IL-17水平各时间点均明显高于N组（P〈O．05）；肺组织和支气管灌洗液中IL-17与肺组织中羟脯氨酸的表达趋势呈负相关（P〈O．05）。结论IL-17参与肺纤维化形成过程。

Effect and significance of IL-17 on bleomycin-induced pulmonary fibrosis in rats

Objective To study the significance and expression of IL-17 in pulmonary fibrosis rats. Methods 64 SD rats were randomly divided into normal control group（N）, pulmonary fibrosis model group（B） and dexametha- sone treated group（D）. Rats in N group and B group were killed on 7th,14th and 28th day,respectively. Rats in D group were killed on 14th day and 28th day,respectively. The levels of IL-17 in bronchial lavage fluid and lung tissue were measured. The pathological changes of lung tissue were observed,and the level of hydroxyproline was detected. Results （1） The lung tissue biopsy in B group showed the dynamic process of change from alveolitis to fibrosis gradually,and the level of hydroxyproline in lung tissue was higher than that in N group and D group. （2） The ex- pression trend of IL-17 in lung tissue was the same as that in bronchial lavage fluid,had peak on 7th day,and lower level expression in N group. These in the B group and D group were significantly higher than these in N group at all the time points（P〈0.05）. IL-17 expression in lung tissue and bronchial tavage fluid was negatively correlated with hydroxyproline expression in lung tissue（P〈0.05）. Conclusion The high expression of IL-17 in B group suggests that it may involve in the formation of pulmonary fibrosis.