中华芦荟多糖对非瘤细胞辐射后细胞凋亡及凋亡蛋白表达的影响

目的　研究中华芦荟多糖 (AP)对X射线照射后人非瘤细胞株CLiver和 2 93细胞凋亡及细胞凋亡蛋白 p5 3,bax ,Bcl 2 ,Bad ,survivin表达的影响。方法　流式细胞仪检测细胞凋亡 ,Westernblot检测细胞凋亡相关蛋白的表达。结果　X射线照射后 72h ,CLiver细胞可见典型的DNA梯形条带 ,照射前加入AP 2 5或 5 0mg·L-1均能使DNA片段减少 ;AP预处理使CLiver细胞在照后 6h和72h凋亡率分别由 5 0 %和 4 3 0 %降至 2 2 %和 10 9% ,使2 93细胞照后 6h凋亡率由 8 6 %降至 4 0 % ;AP预处理可使CLiver细胞p5 3、Bad、Bax蛋白表达量下调 ,Bcl 2和sur vivin蛋白表达则上调。结论　AP对X射线辐射后非瘤细胞凋亡的抑制作用与对凋亡蛋白表达的调节有关

Modulation of Aloe Polysaccharides on X-ray irradiated non malignant cells in apoptosis and apoptotic regulating protein expression

AIM To study the modulation of Aloe Polysaccharides on the apoptosis and apoptotic regulating protein expression in X-ray irradiated non-malignant cells. METHODS The apoptosis was analyzed using flow cytometry. The level of apoptotic regulating protein expression was tested using Western blot. RESULTS A distinct DNA ladder happened in C Liver cells 72 h after irradiation. Pre-treatment of AP in the concentration of 25 or 50 mg·L -1 significantly decreased the DNA ladder. The apoptotic rate of C Liver in 6 h and 72 h after irradiation was decreased from 5.0%, 43.0% to 2.2% and 10.9%, respectively. Meanwhile, pre-treatment of AP down-regulated the expression of p53, Bad and Bax protein caused by irradiation and evidently up-regulated the expression of Bcl-2 and Survivin protein. On the other hand, The apoptotic rate of 293 cells in 6h after irradiation was decreased from 8.6% to 4.0%. CONCLUSION The inhibiting effect of AP on the apoptosis of non-malignant cells was at least partly caused by the modulation of apoptotic regulating protein expression.