原花青素对人胆管癌细胞QBC939抑制的体内外研究

目的研究原花青素(PC)体内外对人胆囊癌细胞的抑制作用。方法常规培养细胞,24 h后随机分为阳性对照组、空白对照组和PC组。MTT法检测PC对人胆囊癌细胞增殖的抑制作用;流式细胞术检测细胞凋亡率和细胞周期;建立QBC939细胞裸鼠异种移植瘤模型。将荷瘤裸鼠随机分为5组:阴性对照组、5-Fu阳性对照组及PC 3个剂量组,每日腹腔注射给药,每隔3 d测肿瘤大小;12 d后,处死裸鼠,剥瘤称重并计算抑瘤率。结果 PC 3个剂量组显著抑制QBC939细胞增殖,且呈时间和剂量依赖性;将细胞周期阻滞在S期,并诱导QBC939细胞凋亡;PC可抑制QBC939细胞裸鼠异种移植瘤的生长。结论 PC体内外均可抑制SHG-44细胞生长,并诱导肿瘤细胞凋亡。

Study on inhibition on human cholangiocarcinoma cell of PC in vivo and in vitro

Objective To study the inhibition effect of PC on human cholangiocarcinoma in vivo and vitro.Methods Cells were cultivated in vitro,and then were divided into positive control group,negative control group and PC group randomly after cultured for 24 h.The cytostatic effect of PC on human cholangiocarcinoma w as evaluated by MTT assay.Flow cytometry w as utilized for measuring the apoptosis and cell cycle.The nude mice w ith QBC939 cell xenografts w ere divided into 5 groups randomly: negative control group,positive control group,PC groups(three dosage groups),and the mice w ere treated w ith drugs via intraperitoneal injection daily.The tumor size w ere measured every three days.After 12 d of treatment,the tumor was weighed,and the inhibitory rate w as calculated.Results PC inhibited cell grow th in a concentration-and-time dependent manner.PC blocked cell cycle in phase S,and elevated the apoptosis rate,PC remarkably inhibited the grow th of QBC939 cell xenografts.Conclusion PC can inhibit the grow th of SHG-44 in vitro and in vivo,and induce the apoptosis of QBC939.