| 野西瓜成熟果实中多糖的含量测定及毛细管电泳分析 |
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| 引用本文： | 季宇彬,郭守东,汲晨锋.野西瓜成熟果实中多糖的含量测定及毛细管电泳分析[J].中国药学杂志,2006,41(15):1186-1189. |
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| 作者姓名： | 季宇彬 郭守东 汲晨锋 |
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| 作者单位： | 哈尔滨商业大学生命科学与环境科学研发中心,哈尔滨,150076;哈尔滨商业大学药物所博士后科研工作站,哈尔滨,150076 |
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| 摘 要： | 目的提取野西瓜成熟果实中的多糖,并对野西瓜果实中多糖进行含量测定及单糖组分分析。方法含量测定采用苯酚-硫酸法;单糖组分分析采用高效毛细管电泳。结果野西瓜粗多糖及除蛋白后(Sevage法)多糖含量分别为39.95%,66.42%;野西瓜多糖的主要单糖组分为:鼠李糖∶木糖∶葡萄糖∶(果糖和甘露糖)∶阿拉伯糖∶半乳糖=3∶5∶48∶100∶21∶19。结论实验中优化后的苯酚硫酸法简便、易行;采用高效毛细管电泳对多糖中单糖组分分析,分离效果也很好。
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| 关 键 词： | 野西瓜 多糖 苯酚-硫酸法 高效毛细管电泳 |
| 文章编号： | 1001-2494（2006）15-1186-04 |
| 收稿时间： | 2005-12-31 |
| 修稿时间： | 2005-12-31 |
Determination and Analysis of Polysaccharide from Capparis spinosa Ripe Fruits by HPCE |
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| JI Yu-bin,GUO Shou-dong,JI Chen-feng.Determination and Analysis of Polysaccharide from Capparis spinosa Ripe Fruits by HPCE[J].Chinese Pharmaceutical Journal,2006,41(15):1186-1189. |
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| Authors: | JI Yu-bin GUO Shou-dong JI Chen-feng |
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| Institution: | a. Center of Research and Development center of Life Sciences and Environmental Sciences ; b . Institute of Materia Medica and Postdoctoral Programme, Harbin University of Commerce, Harbin 150076, China |
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| Abstract: | OBJECTIVE To isolate polysaccharide from Capparis spinosa ripe fruits,determine the content of the polysaccharide and analyze its monosaccharide components.METHODS The contents were deternined by phenol-sulfuric acid method;HPCE was applied to analyze its monosaccharides.RESULTS The content of the coarse polysaccharide and purified polysaccharide(after getting rid of protein by sevage) were 39.95% and 66.42%,respectively.The polysaccharides were mainly composed of Rha∶Xyl∶Glu∶(Fru and Man)∶Ara∶Gal=3∶(5∶48)∶100∶21∶19.CONCLUSION The optimized way of coloration of phenol-sulfuric acid method can be easily controlled and operated;HPCE is a good way to analyze the monosaccharide components of polysaccharides. |
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| Keywords: | Capparis spinosa polysaccharide phenol-sulfuric acid method HPCE |
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