| 黄皮酰胺对糖尿病大鼠海马HO-1和HO-2表达的影响 |
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| 引用本文： | 侯软玲,李炳,魏林郁,梅燕,刘林,林军.黄皮酰胺对糖尿病大鼠海马HO-1和HO-2表达的影响[J].中国药理学通报,2008,24(12). |
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| 作者姓名： | 侯软玲 李炳 魏林郁 梅燕 刘林 林军 |
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| 作者单位： | 1. 新乡医学院生理学与神经生物学教研室,河南,新乡,453003
2. 广西医科大学药学院药理学教研室,广西,南宁,530021 |
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| 基金项目： | 广西壮族自治区硕士研究生科研创新项目资助课题
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河南省教育厅资助课题
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| 摘 要： | 目的探讨黄皮酰胺(Clausenamide,Clau)对糖尿病大鼠海马血红素加氧酶1和2(HO-1和HO-2)表达的影响。方法♂Wistar大鼠腹腔注射链脲佐菌素(48mg·kg-1)建立糖尿病模型,给予不同药物治疗3mon后,分别以RT-PCR法和免疫组织化学法观察大鼠海马HO-1和HO-2 mR-NA和蛋白质的表达。结果①RT-PCR结果显示,糖尿病大鼠海马的HO-1和HO-2 mRNA表达水平明显增加(P<0.01);而Clau(50,25mg·kg-1)治疗组大鼠海马的HO-1和HO-2 mRNA的表达水平明显降低(P<0.01),表明Clau可明显抑制糖尿病大鼠海马HO-1和HO-2 mRNA的表达水平;②免疫组化结果表明,糖尿病大鼠海马HO-1和HO-2的蛋白质表达明显升高(P<0.01),表明HO-1和HO-2蛋白质的异常表达参与了糖尿病障碍的过程,而Clau通过抑制HO-1和HO-2的蛋白质表达对糖尿病大鼠起到保护作用的。结论黄皮酰胺可能通过抑制海马的HO-1和HO-2 mRNA及蛋白质表达起到糖尿病大鼠的海马保护作用的。
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| 关 键 词： | 糖尿病 黄皮酰胺 海马 血红素加氧酶 |
Effects of clausenamide on hippocampus HO-1 and HO-2 expressions in diabetic rats |
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| HOU Ruan-ling,LI Bing,WEI Lin-yu,MEI Yan,LIU Lin,LIN Jun.Effects of clausenamide on hippocampus HO-1 and HO-2 expressions in diabetic rats[J].Chinese Pharmacological Bulletin,2008,24(12). |
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| Authors: | HOU Ruan-ling LI Bing WEI Lin-yu MEI Yan LIU Lin LIN Jun |
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| Institution: | HOU Ruan-ling1,LI Bing1,WEI Lin-yu1,MEI Yan2,LIU Lin2,LIN Jun2(1.Dept of Physiology , Neurobiology,Xinxiang Medical College,Xinxiang Henan 453003,2.Dept of Pharmacology,Guangxi Medical University,Nanning 530021) |
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| Abstract: | Aim To explore the effects of clausenamide(Clau) on hippocampal heme oxygenase(HO-1 and HO-2) expressions in diabetic rats.Methods The diabetic rats model was produced by injecting streptozotocin (48 mg·kg-1). After 3 months,the HO-1 and HO-2 mRNA and protein expressions in hippocampus of diabetic rats were detected by RT-PCR and immunohistochemistry respectively.Results ① The results of RT-PCR showed that the protein expressions of HO-1 and HO-2 mRNA in hippocampus of diabetic group were higher than those of control group(P<0.01).The expressions of HO-1 and HO-2 mRNA of Clau-treated group (50,25 mg·kg-1) were lower than those of diabetic group(P<0.01).② The results of immunohistochemistry showed that the protein expressions of HO-1 and HO-2 in hippocampus of diabetic group were obviously increased(P<0.01),but Clau could inhibit the expressions of HO-1 and HO-2.Conclusion Clau can significantly inhibit HO-1 and HO-2 expressions in hippocampus,which may account for the protective effects. |
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| Keywords: | diabetes mellitus clausenamide hippocampus heme oxygenase |
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