CELLULAR ELEMENTS IN GIANT CELL TUMOR OF BONE
FEULGEN-DNA QUANTITATION, 3H_TdR INCORPORATION AND ULTRASTRUCTURE
OBSERVATION |
| |
Authors: | Y Zhang C H Feng S S Chen J Q Zhang |
| |
Abstract: | Anti-erythrocyte rosette assay was used to sub
divide stromal cells (StCs) of cultured giant cell
tumor (GCT) of bone into two groups, the rosette
forming cells (RFCs) and non-rosette forming cells
(NRFCs). Characteristics of Feulgen-DNA content,
3H.TdR uptake and ultrastructure of different cellular
elemcnts in GCT were then investigated by micro-
spectrophotometry, autoradiography and both TEM
and SEM. Two types of StCs and multinucleated
giant cells (MGCs) showed 2C DNA stem line,
which contrasted strikingly with aneuploid DNA in
osteosarcoma cells. Autoradiography revealed that
the labelling index of NRFCs increased with the time
of 3H.TdR incorporation in vitro, while RFCs and
MGCs were scarcely tagged. The two types of StCs
were distinctly different in both surface and intra-
cellular structures. The phagocytic function and sur-
face appearance of RFCs resembled those of ma
crophages, and no polymorphism was found in RFCs.
These facts suggest that NRFCs are the neoplastic
element in GCT, whereas RFCs are the end-stage
cells and possibly the macrophages related to tumor
immunity. |
| |
Keywords: | |
|
| 点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息 |
| 点击此处可从《中华医学杂志(英文版)》下载免费的PDF全文 |
|