胶质细胞源性神经营养因子在肠易激综合征大鼠模型中的表达

目的:探讨胶质细胞性神经营养因子(GDNF)在肠易激综合征(IBS)大鼠模型中的表达及其作用。方法:45只大鼠随机分为ISB1组、ISB2组及空白对照组,ISB1组、ISB2组分别采用乙酸及慢性激怒的方法制造肠易激综合征大鼠模型,空白对照组不做任何处理,采用免疫组化的方法检测3组大鼠结肠肌间神经丛内GDNF蛋白的表达,并进行比较。结果:ISB1组GDNF表达明显高于ISB2组和空白对照组(P<0.05),而ISB2组与空白对照组GDNF的表达差异无统计学意义(P>0.05)。结论:GD-NF在炎症后肠易激综合征中的表达增加,提示神经元及神经胶质细胞对GDNF需求增加,推测GDNF对结肠肌间神经丛内神经元起保护和促进再生的作用,继而参与调解肠道炎症过程,且可能有保护和促进感染后肠易激综合征肠粘膜恢复的作用。

The expression of glial cell line-derived neurotrophic factor in rat model of irritable bowel syndrome

Objective： To detect the expression of glial cell line-derived neurotrophic factor （GDNF） in the rat model of irritable bowel syndrome. Methods： A total of 45 rat models of irritable bowel syndrome were randomly divided into ISB1, ISB2 and control group. Acetic acid and chronic activation were used to induce irritable bowel syndrome model of ISB1 group and ISB2 group, respectively. Expression of colon myenteric plexus GDNF protein in the three groups was detected and compared with immunohistochemistry method. Results. Expression of GDNF protein was significant higher in ISB1 group than that in ISB2 or the control group （P both〈0. 010）, while comparison between the latter two groups showed no significant differences （P〉0.05）. Conclusions： In the circumstance of iriflammation, expression of GDNF is increased in irritable bowel syndrome models of rat suggesting an increased demand of GDNF for neurons and glial cells. It＇s assumed that GDNF is involved to the development of intestinal inflammation by protecting colonic myenteric plexus neurons and promoting their regeneration, it may also contribute to the recovery of intestinal mucosa from inflammation.