CLONING AND EXPRESSION OF A GENE ASSOCIATED WITH HL<sub>60</sub> CELL APOPTOSISINDUCED BY INHIBITION OF POLYAMINE BIOSYNTHESIS Cloning and expression of a gene associated with HL60 cell apoptosis induced by inhibition of polyamine biosynthesis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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CLONING AND EXPRESSION OF A GENE ASSOCIATED WITH HL₆₀ CELL APOPTOSISINDUCED BY INHIBITION OF POLYAMINE BIOSYNTHESIS

摘要：
Cloning and expression of a gene associated with HL60 cell apoptosis induced by inhibition of polyamine biosynthesis

Authors:	Lixin Feng Muzhen Fan

Institution:	(1) Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Yinghua East Road, Hepingli Street, 100029 Beijing, China

Abstract:	Objective: To clone the gene associated with apoptosis induced by an inhibitor of polyamine biosynthesis, α-difluoromethylornithine (DFMO). Methods: The differential subtraction screening was used for gene cloning from cDNA library of HL₆₀ cells treated by DFMO. Northern blot, morphological observation, FCM assays and ladder map of DNA electrophoresis were performed. Results: The transfecting gene expression and activity of inducing apoptosis in the cells transfected from recombinant plasmid containing the cloned fragment df4 was proved. Conclusion: It is suggest that df4 gene cloned in the study could be a gene regulating apoptosis of HL₆₀ cells.

Keywords:	Polyamines HL₆₀ cell Apoptosis Gene expression DFMO
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