HPLC法测定葛根总黄酮固体脂质纳米粒的载药量及包封率

目的：建立HPLC法同时测定葛根总黄酮固体脂质纳米粒中4种异黄酮类成分的包封率及载药量。方法采用RP-HPLC法，Kromasil C18（4.6mm ×250mm，5μm）色谱柱；甲醇-0.1%枸橼酸溶液为流动相梯度洗脱；流速1.0mL/min，柱温40℃，检测波长250nm。采用高速离心法分离固体脂质纳米粒中游离药物。结果3-羟基葛根素、葛根素、大豆苷和大豆苷元线性关系良好，平均回收率分别为（100.28±2.52）%、（100.26±2.33）%、（100.08±3.35）%及（100.44±3.48）%。3批次葛根总黄酮固体脂质纳米粒中3＇-羟基葛根素、葛根素、大豆苷和大豆苷元的包封率分别为（84.35±0.45）%、（86.84±0.48）%、（89.52±0.86）%及（93.80±0.50）%，其载药量分别为（10.37±0.36）%、（14.19±0.52）%、（16.79±0.34）%及（20.00±0.97）%。结论本法简单快速、结果准确可靠，可同时测定葛根总黄酮固体脂质纳米粒4种成分的载药量与包封率。

Determination of drug loading and entrapment efficiency of pueraria flavonoids loaded solid lipid nanoparticles by HPLC

Objective To establish a method for simultaneous determination of drug loading and entrapment efficiency of four isoflavones in pueraria flavonoids （PF）loaded solid lipid nanoparticles（PF-SLN）. Methods HPLC determination was performed on a Kromasil C18 column （4.6mm×250mm, 5μm） and detected at 250 nm. The mobile phase was consisted of methanol and 0.1% citric acid solution with gradient elution. Flow-rate was 1.0mL/min, and column temperature was 40℃. The free drugs in solid lipid nanoparticles were separated by high-speed centrifuge. Results 3’-hydroxypuerarin, puerarin, daidzin and daidzein had a good linear relation and the average recoveries were （100.28±2.52）%, （100.26±2.33）%, （100.08±3.35）% and （100.44±3.48）%, respectively. The entrapment efficiency of 3’-hydroxypuerarin, puerarin, daidzin and daidzein in PF-SLNs were （84.35±0.45）%, （86.84±0.48）%, （89.52±0.86）% and （93.80±0.50）%, and drug loading were （10.37±0.36）%, （14.19±0.52）%, （16.79±0.34）% and （20.00±0.97）%, respectively. Conclusion Results obtained showed that it’s a convenient, accurate and reliable method. The method has been successfully applied to determination of drug loading and entrapment efficiency of four isoflavonoids in PF-SLNs.