HPLC法测定蛹虫草子实体及其培养基的虫草素含量

目的:建立蛹虫草子实体及其培养基中虫草素(Cordycepin)含量的测定方法。方法:采用高效液相色谱(HPLC)法,选用YMC-PackC18色谱柱(250×4.6mm,5μm),流动相为甲醇-水(20:80),流速为1mL/min,检测波长为260nm,柱温为30℃,分析时间为20min。结果:蛹虫草子实体中虫草素含量为0.503mg/g,平均加样回收率(n=9)为100.8%(RSD=1.33%);蛹虫草培养基中虫草素含量为0.118mg/g,平均加样回收率(n=9)为101.7%(RSD=1.82%)。结论:本方法分离度好、精密度高、专属性强、灵敏度高,可用于蛹虫草子实体及其培养基的质量评价。

Determination of Cordycepin in Cordyceps and its Culture Medium by HPLC

Objective：A new HPLC method was developed to determine the content of the Cordycepin in fruitbodies as well as Culture medium.Methods：HPLC was performed on a YMC-Pack C18 reversed-phase column（250×4.6mm,5μm） .All of the reference substances and sample were separated with the mobile phase of methanol：water（20：80） under isocratic elution for 20min,flow rate was 1.0mL/min,the detection wavelength was 260nm,and the column temperature was 30℃.Results：The content of the Cordycepin in the fruitbody were 0.503mg/g and 0.118mg/g in the Culture medium.The average recovery rate were about 101.1%（n=9,RSD=1.33%） in the fruitbody and 101.7%（n=9,RSD=1.82%） in the Culture medium.Conclusion：This developed method,which is simple and with high sensitivity as well as selectivity,can be used for quality evaluation of the Cordyceps.