| 中药虎杖雌激素活性组分的分离及活性测试 |
| |
| 引用本文： | 张彩宁,王煦漫,张晓哲,肖红斌,梁鑫淼. 中药虎杖雌激素活性组分的分离及活性测试[J]. 世界科学技术, 2007, 9(2): 58-64 |
| |
| 作者姓名： | 张彩宁 王煦漫 张晓哲 肖红斌 梁鑫淼 |
| |
| 作者单位： | [1]西安工程大学纺织与材料学院,西安710048 [2]中国科学院大连化学物理研究所,大连116023 |
| |
| 基金项目： | 中国科学院知识创新工程项目 |
| |
| 摘 要： | 药材虎杖用70％乙醇冷浸法进行提取。对虎杖中蒽醌类化合物集中分布的乙酸乙酯分配组分首先进行硅胶柱分离，得到6个组分，利用转基因酵母法测定其雌激素活性，结果表明前三个组分具有较高的雌激素活性。进一步对其中的第三个活性组分利用制备高效液相色谱进行了精制，对流出物按2分钟间隔收集，共得到20个馏分。转基因酵母法测定其雌激素活性的结果显示，在这20个馏分当中，有5个馏分具有较强的雌激素活性。分别将其雌激素活性与化学组成进行关联，发现其中的sub-fraction-13中可能存在非大黄素的雌激素活性成分。
|
| 关 键 词： | 虎杖 制备高效液相色谱 雌激素活性 转基因酵母 |
| 收稿时间： | 2006-12-05 |
| 修稿时间： | 2007-03-12 |
Preparation and Bioassay of Estrogenic Compounds in Polygonum cuspidatum |
| |
| Zhang Caining,Wang Xuman,Zhang Xiaozhe,Xiao Hongbin,Liang Xinmiao. Preparation and Bioassay of Estrogenic Compounds in Polygonum cuspidatum[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2007, 9(2): 58-64 |
| |
| Authors: | Zhang Caining Wang Xuman Zhang Xiaozhe Xiao Hongbin Liang Xinmiao |
| |
| Abstract: | In this study, the Chinese herb - Polygonum cuspidatum was extracted using a 70% ethanol. The ethyl acetate fraction was isolated from the extract using silica gel column chromatography. Total six fractions were obtained. The recombinant yeast cells were used to determine estrogenic activities of the fractions. Experimental results showed that three fractions had a higher estrogenic activity, compared with the rest of fractions. Preparative high performance liquid chromatography was then employed to separate the active compound Hz001 - 03, from which 20 sub - fractions were produced. Among them, five sub - fractions were found with a higher estrogenic activity. The results of both bioassay and HPLC analysis have led to the conclusion that unknown bioactive compounds might exist in sub -fraction- 13. |
| |
| Keywords: | Polygonum cuspidatum preparative high performance liquid chromatography estrogenic activity recombinant yeast |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
