| 醇沉对纯化金银花中绿原酸工艺的影响 |
| |
| 引用本文： | 杨海燕,陈福明,阎武. 醇沉对纯化金银花中绿原酸工艺的影响[J]. 中成药, 2006, 28(9): 1256-1259 |
| |
| 作者姓名： | 杨海燕 陈福明 阎武 |
| |
| 作者单位： | 1. 深圳清华大学研究院新材料与生物医药研究所,广东,深圳,518057
2. 珠海清华科技园创业投资有限公司,广东,珠海,519080 |
| |
| 基金项目： | 珠海科技局资助项目(PB20041017) |
| |
| 摘 要： | 目的:测定醇沉对绿原酸纯化工艺的影响。方法:采用HPLC测定方法对不同醇沉度提取金银花粗粉中绿原酸的效果进行了比较。结果:最佳醇沉度为60。采用醇沉、萃取复合工艺可将粗粉中绿原酸含量从5.5提高到37.72,收率为83.53。结论:醇沉可有效去除金银花粗粉中的杂质,且绿原酸收率高达90.16。
|
| 关 键 词： | 绿原酸 金银花 醇沉 纯化 |
| 文章编号： | 1001-1528（2006）09-1256-03 |
| 收稿时间： | 2005-08-18 |
| 修稿时间： | 2005-08-18 |
Effect of ethanol precipitation on chlorogenic acid from Flos Lonicerae Japonica |
| |
| YANG Hai-yan,CHEN Fu-ming,YAN Wu. Effect of ethanol precipitation on chlorogenic acid from Flos Lonicerae Japonica[J]. Chinese Traditional Patent Medicine, 2006, 28(9): 1256-1259 |
| |
| Authors: | YANG Hai-yan CHEN Fu-ming YAN Wu |
| |
| Abstract: | AIM: To test the effect of ethanol precipitation on the purifying technology of chlorogenic acid (CGA). METHODS: Using HPLC, the effect of the extraction of CGA from the crude Flos Lonicerae Japonica powder with three concentrations of ethanol was compared. RESULTS: Ethanol with concentration of 60% was optimal. CGA content was raised from 5.5% to 37.72% by use of ethanol precipitation and extraction in combination. CONCLUSION: The ethanol precipitation can get rid of the impurity in the crude powder of Flos Lonicerae Japonica effectively and the yield of CGA can reach 90.16%. |
| |
| Keywords: | chlorogenic acid Flos Lonicerae Japonica ethanol precipitation purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
