磁性纳米颗粒介导基因治疗乳腺癌实验研究

 目的 探讨新型基因载体PEI包裹的磁性纳米颗粒polyMAG-1000介导TRAIL基因治疗乳腺癌的可行性，观察TRAIL基因转染乳腺癌细胞后的治疗作用。方法 以polyMAG-1000为基因载体，联结TRAIL质粒DNA后转染人乳腺癌细胞株MCF-7细胞，用Tunel法检测细胞的凋亡，用流式细胞仪检测细胞的凋亡率，以空载体作为阴性对照；脂质体转染TRAIL基因作阳性对照。结果 Tunel法可见MCF-7细胞经polyMAG1000和脂质体转染TRAIL基因后均可见发生凋亡的细胞，流式细胞仪检测polyMAG-1000转染的细胞凋亡率为25．11％±2．85％，脂质体转染的细胞凋亡率为18．31％±2．44％（P〈0．05）。结论 TRAlL对乳腺癌细胞MCF-7具有凋亡效应，PEI包裹的磁性纳米颗粒介导的TRAIL基因治疗在肿瘤的基因治疗中具有应用前景。

Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy-A Study in Vitro on Breast Cancer

Objective 　To study the feasibility of using TRAIL gene to treat breast cancer mediated by PEI coated magnetic iron oxide nanoparticles (polyMA G-1000) . The therapeutic efficacy was also be evaluated. Methods 　PEI coated magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMA G-1000 without TRAIL gene was t ransfected into the tumor cells as negative cont rol and liposome mediated TRAIL gene t ransfection was taken as positive cont rol. The apoptosis of cells were detected by TUNEL method. The apoptosis ratio of tumor cells were measured with flow cytometry ( FCM) . Results 　The apoptosis of cells was demonst rated after transfecting TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMA G-1000 as gene carrier were 25. 11 % ±2. 85 % , whereas it was 5. 06 % ±1. 05 % in the cont rol group with polyMA G-1000 ( P < 0. 01) . The lower apoptosis ratio of 18. 31 % ±2. 44 % was showed in the group with liposome as gene carrier ( P < 0. 05 ,compared with the group with polyMA G-1000 as gene carrier) . Conclusion 　TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The PEI coated magnetic iron oxide nanoparticles may be a potential gene carrier with high transfection efficacy in cancer gene therapy.