视黄酸受体抑动剂对正常人体T淋巴细胞增殖的影响

目的]评价视黄酸受体RARs特异性抑动剂BIBn和视黄酸受体RXRs特异性抑动剂HX603对正常成年人体T淋巴细胞的增殖的影响,确定剂量水平,建立一套通过受体途径研究维生素A功能的实验方法。方法]用密度梯度分离法和磁性细胞分选法从健康人的外周血分离T淋巴细胞,进行体外培养,采用MTT法,绘制人T淋巴细胞的体外生长曲线,并测定在10-4mol/L、10-5mol/L、10-6mol/L、10-7mol/L4个不同浓度时的OD值,用方差检验分析各剂量对细胞的影响。结果]在培养24h时1×10-4mol/L、1×10-5mol/L、1×10-6mol/L、1×10-7mol/L浓度的BIBn剂量组结果OD值分别为0.538±0.128、0.692±0.068、0.627±0.136、0.730±0.157(P﹤0.05)。结论]在培养24h时BIBn对T淋巴细胞增殖有抑制作用。HX603对T淋巴细胞增殖可能有抑制作用。

THE EFFECT OF RETINOIC ACID RECEPTOR ANTAGONIST ON REGULAR HUMAN T CELLS

Objective]To evaluate the effects of BIBn(the RARs specific antagonist)and HX603(the RXRs specific antagonist)on proliferation of T-Lymphocytes of human,identify the dose status,and establish a method which is dependent on retinoic acid receptor pathway to study the functions of vitamin A.Methods]T-Lymphocytes were separated from the blood of healthy people by the way of the density gradient separation and MACS,then cultured in vitro.MTT method was used to draw the growth curve and measure the OD value at...