The expression and anatomical distribution of BTLA and its ligand HVEM in rheumatoid synovium |
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Authors: | Shang Yongjun Guo Guoning Cui Qifu Li Jinlong Ruan Zhihua Chen Yongwen |
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Institution: | (1) Department of Orthopedics, Affiliated Hospital of Chifeng University, Inner Mongolia, 024000, People’s Republic of China;(2) Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People’s Republic of China;(3) Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People’s Republic of China;(4) Institute of Immunology, Third Military Medical University, Chongqing, 400038, People’s Republic of China; |
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Abstract: | Co-inhibitory signaling from B and T lymphocyte attenuator (BTLA) can suppress lymphocyte activation and maintain peripheral
tolerance. However, the expression and anatomical distribution of BTLA and its ligand, herpesvirus entry mediator (HVEM),
in rheumatoid arthritis (RA) synovium have not been reported. In this study, we analyzed the expression of HVEM and BTLA in
RA synovium by immunohistochemistry, and our results showed that both factors were observed in all four cases of RA samples.
At the cellular level, both HVEM and BTLA were found on the cell membrane and in the cytoplasm. Fluorescence dual staining
demonstrated that HVEM was chiefly on CD3+ T cells, CD68+ macrophages, and to a lesser extent was found on CD31+ endothelial cells. Similarly, the expression of BTLA was observed on infiltrated CD3+ T cells and CD68+ macrophages. The co-expression of HVEM and BTLA with some members of the B7 family in these sections was also analyzed, and
the results showed that HVEM antigen was also found on B7-H3+ capillaries, while it was absent on B7-H1+, B7-DC+, B7-H4+, and Z39Ig+ cells. Interestingly, BTLA was observed on B7-H1+, B7-H4+, and HVEM+ cells in the synovium. The characteristic expression and distribution of BTLA/HVEM in the synovium indicated that their signaling
probably affects the pathogenesis of RA, and a clear understanding of their functional roles may further elucidate the pathogenesis
of this disease. |
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