二氧化锆梯度复合羟基磷灰石生物陶瓷的制备及其体外免疫相容性

制备二氧化锆（ZrO2）梯度复合羟基磷灰石（HAP）生物陶瓷材料、ZrO2单纯复合HAP陶瓷材料及两种材料浸提液。检测梯度复合材料的截断面特征；在有及无植物血凝素（PHA）的情况下，检测材料浸提液对健康青年人末梢血单个核细胞（PBMC）的活化。结果表明：PHA作用72h，梯度复合组PBMC的增殖作用高于单纯复合组（P〈0．05）；两材料对PBMC的凋亡无差异（P〉0．05）；PHA作用24h，两材料对CD3／CD69表达率上差异均有统计意义（P〈0．01）。ZrO2梯度复合HAP生物陶瓷材料对PBMC的激活程度较轻，梯度复合技术有助于提高ZrO2-HAP生物陶瓷的免疫相容性。

Preparation of Graded Zirconia-hydroxyapatite Composite Bioceramic And Its Immunocompatibility In Vitro

Graded Zirconia-hydroxyapatite composite bioceramic and simplex Zirconia-hydroxyapatite composite bioceramic were produced,and the extractes of these two of materials were made to evaluate their immunocompatibility.Scanning electron microscope(SEM) was used to detect the character of the surface of the graded composite bioceramic.Holding and without holding phytohemagglutinin(PHA),proliferation and activation of peripheralblood monocytes(PBMCs) cultured in the two extracts were studied.Cultured in PHA after 72 hours,the proliforation rate of the graded composite group was significantly higher than the simplex composite group(P<0.01).There was no difference of apoptosis of PBMCs of the two groups(P>0.05).Cultured in PHA after 24 hours,the ratio of CD3/CD69 positive PBMCs of the simple composite group was significantly higher than that of the graded composite material group(P<0.01).The numbers of PBMCs activated by the graded composite material group were less than that of the simply composite material group and the technique of graded composite will be helpful to improve its immunocompatibility.