高效液相色谱手性流动相添加剂法测定富马酸(R，R)-戊乙奎醚中3个光学异构体杂质的含量

 目的 建立拆分富马酸(R，R)-戊乙奎醚与其3个光学异构体的高效液相色谱分离方法，控制光学纯度。方法 选用C₁₈(4.6 mm×250 mm，5 μm)色谱柱，以乙腈-0.05 mol·L^-1磷酸二氢钾（含0.6%三乙胺和0.015 mol·L^-1β-环糊精，pH为2.3）为流动相，柱温25 ℃，检测波长206 nm，流速1.0 mL·min^-1。结果 3个光学异构体的检测灵敏度为9.0 ng，本方法可检出限量为0.09%的光学异构体杂质。结论 本法可有效分离富马酸(R，R)-戊乙奎醚及其3个光学异构体，可用于控制本品光学纯度。

Simultaneous Determination of Five Index Components in Glycyrrhizae Radix et Rhizoma and Longchai Decoction by HPLC

OBJECTIVE To establish a determination method of five index components to control the quality of Glycyrrhizae Radix et Rhizoma and Longchai Decoction. METHODS An ODS C₁₈ column (4.6 mm×250 mm， 5 μm) was adopted； the mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at the flow rate of 1 mL·min^-1；the detection wavelength was 237 nm，and the column temperature was 30 ℃.RESULTS The five index components achieved baseline separation， and the negative sample showed no interference. The linear ranges were 0.156 8-1.568 μg for liquiritin (r=0.999 7)， 0.157 1-1.571 μg for isoliquiritin （r=0.999 8）， 0.155 8-1.558 μg for liquiritigenin (r=0.999 6)， 0.187 1-1.871 μg for glycyrrhizic（r=0.996 9） and 0.158 1-1.58 1 μg for isoliquiritigenin (r=0.999 5)， respectively. The average recoveries were 101.47%（RSD=1.272%）， 101.09%（RSD=1.937%）， 101.14%（RSD=2.388%）， 100.38%（RSD=1.448%）and 100.86%（RSD=1.759%）， respectively (n=5). CONCLUSION This method has good resolution and high precision， and can be used for the quality control of Glycyrrhizae Radix et Rhizoma and Longchai decoction.