宫颈癌患者外周血T淋巴细胞亚群和NK细胞数量与临床分期的相关性分析

目的 探讨宫颈癌患者外周血T淋巴细胞亚群和NK细胞数量的变化及其与宫颈癌分期的相关性.方法 收集2010年6月-2011年6月期间在沈阳医学院奉天医院妇科治疗的146例宫颈癌患者及同期在我院进行体检的50例健康女性(对照组),宫颈癌患者根据临床分期分为0期、Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期5组.采用免疫荧光法和流式细胞仪测定各组患者外周血T淋巴细胞亚群和NK细胞数量.结果 宫颈癌患者CD3+,CD3+ CD4+,NK细胞数量及CD3+ CD4+/CD3+ CD8+比值均低于对照组,且随分期增加而逐渐降低；而CD3+ CD8+细胞数量高于对照组,且随分期增加而逐渐增高.CD3+、CD3+CD4+、NK细胞数量及CD3+ CD4+/CD3+ CD8+细胞比值与宫颈癌临床分期呈负相关(P＜0.01)；CD3+ CD8+细胞数量与宫颈癌分期呈正相关(P＜0.01).结论 宫颈癌患者细胞免疫功能低下,且临床分期越晚,免疫功能越低,检测T淋巴细胞亚群、NK细胞可用于宫颈癌患者的免疫监测.

Correlation Analysis of T Lymphocyte Subsets and NK Cell in Peripheral Blood and Clinical Stage of Cervical Cancer

ObjectiveTo investigate the numbers of T lymphocyte subset and NK cell and the correlation to clinical stages of cervical cancer in the patients with cervical cancer.Methods146 patients with cervical cancer and 50 healthy women from June 2010 to June 2011,were selected.According to the clinical stages,patients were divided into 5 groups:stage 0,stage Ⅰ,stage Ⅱ,stage Ⅲ,and stage Ⅳ group.Immunofluorescence and flow cytometry were used to detect the numbers of T lymphocyte subsets and NK cells in blood samples from every group.ResultsCD3⁺,CD3⁺CD4⁺ T cells,NK cells and CD3⁺CD4⁺/CD3⁺CD8⁺ ratio in patients with cervical cancer were lower than those in the healthy control,and those were negative correlated to clinical stages,namely were decreased with the increasing of clinical stages.CD3⁺CD8⁺ T lymphocyte in patients with cervical cancer was higher than that in the healthy control,and it was increased with the increasing of clinical stage of cervical cancer,mainly was positive correlated with clinical stages of cervical cancer(P＜0.01).ConclusionThe cellular immune function of the patients with cervical cancer was lower and even worse in advanced clinical stages.It is valuable to estimate the function of cell immune by detecting T lymphocyte subset and NK cell.